Anti-CSDE1/NRU 抗体 (ab96124)
Key features and details
- Rabbit polyclonal to CSDE1/NRU
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-CSDE1/NRU antibody
CSDE1/NRU 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CSDE1/NRU -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant fragment corresponding to Human CSDE1/NRU aa 167-372. (AAH32446)
-
ポジティブ・コントロール
- HeLa whole cell lysate (ab150035); A549, HepG2 and HCT116 lysates
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96124の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 88 kDa.
|
|
IHC-P | (1) |
1/100 - 1/1000.
|
ICC/IF |
1/100 - 1/1000.
|
特記事項 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 88 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
-
機能
RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. -
配列類似性
Contains 9 CSD (cold-shock) domains. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 513993 Cow
- Entrez Gene: 7812 Human
- Entrez Gene: 229663 Mouse
- Entrez Gene: 117180 Rat
- Omim: 191510 Human
- SwissProt: O75534 Human
- SwissProt: Q91W50 Mouse
- SwissProt: P18395 Rat
see all -
別名
- Cold shock domain containing E1 RNA binding antibody
- Cold shock domain containing protein E1 antibody
- Cold shock domain-containing protein E1 antibody
see all
画像
-
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ab96124 at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min -
All lanes : Anti-CSDE1/NRU antibody (ab96124) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : A375 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : The HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 88 kDa -
Immunofluorescence analysis of methanol-fixed HeLa, using ab96124 antibody at 1:200 dilution. Costained with Hoechst 33342.
-
Anti-CSDE1/NRU antibody (ab96124) at 1/3000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 88 kDa
7.5% SDS PAGE
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab96124 は 3 報の論文で使用されています。
- Martinez-Useros J et al. UNR/CSDE1 Expression Is Critical to Maintain Invasive Phenotype of Colorectal Cancer through Regulation of c-MYC and Epithelial-to-Mesenchymal Transition. J Clin Med 8:N/A (2019). PubMed: 31027221
- Martinez-Useros J et al. UNR/CDSE1 expression as prognosis biomarker in resectable pancreatic ductal adenocarcinoma patients: A proof-of-concept. PLoS One 12:e0182044 (2017). PubMed: 28763470
- Ju Lee H et al. A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells. Nat Commun 8:1456 (2017). PubMed: 29129916