リコンビナントRabMAb

Anti-CRTAM 抗体 [EPR23786-12] (ab272723)

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Flow Cytometry - Anti-CRTAM antibody [EPR23786-12] (ab272723)
  • Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
  • Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
  • Anti-CRTAM antibody [EPR23786-12] (ab272723)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23786-12] to CRTAM
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-CRTAM antibody [EPR23786-12]
    CRTAM 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23786-12] to CRTAM

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse thymus tissue lysate; Rat cerebellum tissue lysate; Human PBMC (untreated and treated with 1ng/mL phorbol-12-myristate-13-acetate and 0.2 µM ionomycin for 5 hours) whole cell lysates. Flow Cyt: Human PBMCs.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR23786-12

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
ICC
Human
IHC-P
Mouse
Rat
Human
IP
Human
WB
Mouse
Rat
Human
アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 45 kDa).
Flow Cyt
1/500.
特記事項
WB
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 45 kDa).
Flow Cyt
1/500.
  • 追加情報
    Is unsuitable for ICC/IF,IHC-P or IP.

    • ターゲット情報

    • 機能

      Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo.

    • 組織特異性

      In the immune system, expression is restricted to activated class-I MHC-restricted cells, including NKT and CD8 cells. Strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in Purkinje neurons in cerebellum. Expressed at much lower levels in testis, ovary, colon, lung and lymphoid tissues.

    • 配列類似性

      Belongs to the nectin family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • CD355 antibody
      • Class I MHC restricted T cell associated molecule antibody
      • Class-I MHC-restricted T-cell-associated molecule antibody
      • CRTAM antibody
      • CRTAM_HUMAN antibody
      • Cytotoxic and regulatory T cell molecule antibody
      • Cytotoxic and regulatory T-cell molecule antibody
      see all

    画像

    • Flow Cytometry - Anti-CRTAM antibody [EPR23786-12] (ab272723)
      Flow Cytometry - Anti-CRTAM antibody [EPR23786-12] (ab272723)

      Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) (treated with 1 ng/mL PMA and 0.2 μM Ionomycin for 5h) labelling CRTAM with ab272723 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

      Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Cells were stained with rabbit IgG or ab272723. Then stained with anti-CD8 alpha conjugated to Pacific blue.

      Gated on viable cells.

    • Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
      Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
      All lanes : Anti-CRTAM antibody [EPR23786-12] (ab272723) at 1/1000 dilution

      Lane 1 : Untreated human peripheral blood mononuclear cell (PBMC) whole cell lysate with NFDM/TBST
      Lane 2 : Human PBMC treated with 1ng/mL phorbol-12-myristate-13-acetate (PMA) (ab120297) and 0.2 µM ionomycin (ab120116) for 5 hours, whole cell lysate with NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Blocking peptides at 5 % per lane.

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody)

      Predicted band size: 45 kDa
      Observed band size: 70/85 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      The molecular weight observed is consistent with what has been described in the literature (PMID:10811014, 23637756, 16300832) .

    • Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
      Western blot - Anti-CRTAM antibody [EPR23786-12] (ab272723)
      All lanes : Anti-CRTAM antibody [EPR23786-12] (ab272723) at 1/1000 dilution

      Lane 1 : Mouse thymus tissue lysate with NFDM/TBST
      Lane 2 : Rat cerebellum tissue lysate with NFDM/TBST

      Lysates/proteins at 40 µg per lane.

      Blocking peptides at 5 % per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

      Predicted band size: 45 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      The molecular weight observed is consistent with what has been described in the literature (PMID:10811014, 23637756, 16300832).

    • Anti-CRTAM antibody [EPR23786-12] (ab272723)
      Anti-CRTAM antibody [EPR23786-12] (ab272723)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272723 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272723 は論文での使用が確認できていません。

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