Anti-CRTAM 抗体 [EPR23786-12] (ab272723)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23786-12] to CRTAM
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-CRTAM antibody [EPR23786-12]
CRTAM 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23786-12] to CRTAM -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cytmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Mouse thymus tissue lysate; Rat cerebellum tissue lysate; Human PBMC (untreated and treated with 1ng/mL phorbol-12-myristate-13-acetate and 0.2 µM ionomycin for 5 hours) whole cell lysates. Flow Cyt: Human PBMCs.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23786-12 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272723の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 45 kDa).
|
|
Flow Cyt |
1/500.
|
特記事項 |
---|
WB
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 45 kDa). |
Flow Cyt
1/500. |
ターゲット情報
-
機能
Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. -
組織特異性
In the immune system, expression is restricted to activated class-I MHC-restricted cells, including NKT and CD8 cells. Strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in Purkinje neurons in cerebellum. Expressed at much lower levels in testis, ovary, colon, lung and lymphoid tissues. -
配列類似性
Belongs to the nectin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 56253 Human
- Entrez Gene: 54698 Mouse
- Entrez Gene: 300649 Rat
- GenBank: BC070266.1 Human
- Omim: 612597 Human
- SwissProt: O95727 Human
- SwissProt: Q149L7 Mouse
- Unigene: 159523 Human
see all -
別名
- CD355 antibody
- Class I MHC restricted T cell associated molecule antibody
- Class-I MHC-restricted T-cell-associated molecule antibody
see all
画像
-
Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) (treated with 1 ng/mL PMA and 0.2 μM Ionomycin for 5h) labelling CRTAM with ab272723 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or ab272723. Then stained with anti-CD8 alpha conjugated to Pacific blue.
Gated on viable cells.
-
All lanes : Anti-CRTAM antibody [EPR23786-12] (ab272723) at 1/1000 dilution
Lane 1 : Untreated human peripheral blood mononuclear cell (PBMC) whole cell lysate with NFDM/TBST
Lane 2 : Human PBMC treated with 1ng/mL phorbol-12-myristate-13-acetate (PMA) (ab120297) and 0.2 µM ionomycin (ab120116) for 5 hours, whole cell lysate with NFDM/TBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody)
Predicted band size: 45 kDa
Observed band size: 70,85 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe molecular weight observed is consistent with what has been described in the literature (PMID:10811014, 23637756, 16300832) .
-
All lanes : Anti-CRTAM antibody [EPR23786-12] (ab272723) at 1/1000 dilution
Lane 1 : Mouse thymus tissue lysate with NFDM/TBST
Lane 2 : Rat cerebellum tissue lysate with NFDM/TBST
Lysates/proteins at 40 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe molecular weight observed is consistent with what has been described in the literature (PMID:10811014, 23637756, 16300832).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (1)
ab272723 は 1 報の論文で使用されています。
- He X et al. Identification and Validation of Hub Genes for Predicting Treatment Targets and Immune Landscape in Rheumatoid Arthritis. Biomed Res Int 2022:8023779 (2022). PubMed: 36317112