Anti-CRM1 抗体 (ab3459)

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Western blot - Anti-CRM1 antibody (ab3459)

    Key features and details

    • Rabbit polyclonal to CRM1
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-CRM1 antibody
      CRM1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to CRM1

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Mouse, Human

    • 免疫原

      Synthetic peptide corresponding to Human CRM1 aa 1043-1058.
      Sequence:

      RQADEEKHKRQMSVPGS


      (Peptide available as ab41768)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 一次抗体 備考

      Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC’s. NPC’s contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Active transport of molecules through the NPC is a signal-mediated process that is not well understood. Proteins to be exported from the nucleus to cytoplasm contain leucine-rich nuclear export signals (NESs). NESs are recognized by transport receptors belonging to the importin-beta family of proteins. It has been shown that NES export is mediated by CRM 1 (Chromosome Region Maintenance 1), an importin-beta family member that binds NESs, Ran:GTP, and Ran binding protein (RanBP). CRM 1 mediates the nuclear exportation of numerous proteins, including histone deacetylases (HDACs), viral proteins, immunophilins, and STAT 1.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3459の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/500 - 1/5000. Detects a band of approximately 135 kDa (predicted molecular weight: 119 kDa).
    特記事項
    WB
    1/500 - 1/5000. Detects a band of approximately 135 kDa (predicted molecular weight: 119 kDa).

    ターゲット情報

    • 機能

      Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.

    • 組織特異性

      Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.

    • 配列類似性

      Belongs to the exportin family.
      Contains 10 HEAT repeats.
      Contains 1 importin N-terminal domain.

    • 翻訳後修飾

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • 細胞内局在

      Cytoplasm. Nucleus > nucleoplasm. Nucleus > Cajal body. Nucleus > nucleolus. Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Chromosome region maintenance 1 protein homolog antibody
        • CRM 1 antibody
        • CRM1 homolog antibody
        • DKFZp686B1823 antibody
        • emb antibody
        • Exp 1 antibody
        • Exp1 antibody
        • Exportin 1 antibody
        • Exportin-1 antibody
        • Exportin1 antibody
        • XPO 1 antibody
        • xpo1 antibody
        • XPO1_HUMAN antibody
        see all

      画像

      • Western blot - Anti-CRM1 antibody (ab3459)
        Western blot - Anti-CRM1 antibody (ab3459)
        All lanes : Anti-CRM1 antibody (ab3459) at 1/1000 dilution

        Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate
        Lane 2 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
        Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

        Lysates/proteins at 25 µg per lane.

        Secondary
        All lanes : HRP-conjugated anti-rabbit

        Predicted band size: 119 kDa
        Observed band size: 130 kDa why is the actual band size different from the predicted?

      参考文献 (4)

      ab3459 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3459 は 4 報の論文で使用されています。

      • Lussi YC  et al. The nucleoporin Nup88 is interacting with nuclear lamin A. Mol Biol Cell 22:1080-90 (2011). PubMed: 21289091
      • Castanotto D  et al. CRM1 mediates nuclear-cytoplasmic shuttling of mature microRNAs. Proc Natl Acad Sci U S A 106:21655-9 (2009). IP ; Human . PubMed: 19955415
      • Lai MC  et al. The DEAD-box RNA helicase DDX3 associates with export messenger ribonucleoproteins as well as tip-associated protein and participates in translational control. Mol Biol Cell 19:3847-58 (2008). ICC/IF ; Human . PubMed: 18596238
      • Mostaqul Huq MD  et al. Suppression of receptor interacting protein 140 repressive activity by protein arginine methylation. EMBO J 25:5094-104 (2006). WB . PubMed: 17053781

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      Immunocytochemistry abreview for Anti-CRM1 antibody

       Excellent
      Abreviews
      Application
      Immunocytochemistry
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
      Read More

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      投稿 Sep 06 2006

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