Anti-CRISPR-Cas9 抗体 [KANI345B] - BSA and Azide free (ab271303)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [KANI345B] to CRISPR-Cas9 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC
Related conjugates and formulations
製品の概要
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製品名
Anti-CRISPR-Cas9 antibody [KANI345B] - BSA and Azide free
CRISPR-Cas9 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [KANI345B] to CRISPR-Cas9 - BSA and Azide free -
由来種
Rat -
アプリケーション
適用あり: WB, IHC-P, ICCmore details -
種交差性
交差が予測される動物種: Streptococcus pyogenes -
免疫原
Recombinant full length protein. This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- WB: HEK-293 transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag, whole cell lysate. IHC-P: HEK-293T transfected with a GFP-Myc-tagged CRISPR-associated endonuclease Cas9/Csn1 construct. ICC: HEK-293T cells transfected with GFP-tagged Cas9 expression vector.
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特記事項
ab271303 is the carrier-free version of ab271293.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
KANI345B -
アイソタイプ
IgG2a
関連製品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab271303の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
Use at an assay dependent concentration. |
ターゲット情報
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関連性
[FUNCTION] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself. -
参照データベース
- Entrez Gene: 901176 Streptococcus pyogenes
- SwissProt: Q99ZW2 Streptococcus pyogenes
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別名
- Cas9 antibody
- CRISPR-associated endonuclease Cas9/Csn1 antibody
- CRISPR-Cas9/Csn1 antibody
see all
画像
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All lanes : Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293) at 1/5000 dilution
Lane 1 : HEK-293 (human embryonic kidney epithelial cell) transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag, whole cell lysate
Lane 2 : HEK-293T transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 3 : HEK-293 transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag, whole cell lysate
Lane 4 : HEK-293 transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus) with Myc-His tag, whole cell lysate
Lane 5 : HEK-293 transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag, whole cell lysate
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Exposure time: 1 secondThis image was produced using ab271293, the same antibody clone but in a different formulation.
Blocking/Dilution buffer: 5% NFDM/TBST.
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This image was produced using ab271293, the same antibody clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded (Panel A) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GFP-Myc-tagged CRISPR-associated endonuclease Cas9/Csn1 construct and (Panel B) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with empty plasmid, labeling CRISPR-Cas9 with ab271293 at 1/200 (4.58 ug/ml) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with Hematoxylin.
Positive staining on (A) HEK-293T transfected with a GFP-Myc-tagged CRISPR-associated endonuclease Cas9/Csn1 construct, no staining on (B) HEK-293T transfected with empty plasmid.Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
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This image was produced using ab271293, the same antibody clone but in a different formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T+OE-94 cells labelling CRISPR-Cas9 with ab271293 at 1/50 dilution (18.32 ug/ml), followed by ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (2 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing cytoplasmic staining in 293T cells transfected with GFP-tagged Cas9 expression vector.
Secondary antibody only control: Secondary antibody is ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (2 ug/ml). -
All lanes : Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293) at 1/5000 dilution
Lane 1 : HEK-293 (human embryonic kidney epithelial cell) transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag, whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : C6 (rat glial tumor glial cell), whole cell lysate
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Exposure time: 1 secondThis image was produced using ab271293, the same antibody clone but in a different formulation.
Blocking/Dilution buffer: 5% NFDM/TBST.
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This image was produced using ab271293, the same antibody clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CRISPR-Cas9 with ab271293 at 1/200 (4.58 ug/ml) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Negative control: No staining on the human tonsil. -
This image was produced using ab271293, the same antibody clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CRISPR-Cas9 with ab271293 at 1/200 (4.58 ug/ml) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Negative control: No staining on the mouse spleen. -
This image was produced using ab271293, the same antibody clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CRISPR-Cas9 with ab271293 at 1/200 (4.58 ug/ml) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Negative control: No staining on the rat spleen.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab271303 は論文での使用が確認できていません。