Creatinine Assay Kit (Colorimetric) (ab204537)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Urine
製品の概要
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製品名
Creatinine Assay Kit (Colorimetric)
Creatinine キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% 7.981 mg/dl 20 = 2.159% 7.452 mg/dl 20 = 3.203% 2.007 mg/dl 20 = 5.995% 7.326 mg/dl 20 = 3.729% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% 7.591 mg/dl 20 = 2.249% 7.232 mg/dl 20 = 2.052% 1.932 mg/dl 20 = 2.027% 7.085 mg/dl 20 = 1.433% -
サンプルの種類
Urine -
アッセイタイプ
Quantitative -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Urine = 95 92% - 99% -
全工程の試験時間
0h 30m -
種交差性
交差種: Mammals, Other species -
製品の概要
Abcam's Creatinine Assay Kit (Colorimetric) (ab204537) is a complete kit for the quantitative determination of creatinine in urine, and is based upon the Jaffe reaction.
Sensitivity = 0.042 mg/dL.
General range = 0.31 - 20 mg/dL
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特記事項
Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or is synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and p-creatine are converted nonenzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine, and under normal conditions, its formation occurs at a relatively constant rate. Intra-individual variation of creatinine levels is <15% from day to day, making it a useful marker for normalizing levels of other molecules found in urine. Altered creatinine levels may be associated with conditions that result in decreased renal blood flow, such as diabetes and cardiovascular disease.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 2 x 96 tests Clear Microtiter Plates 2 units Creatinine Detection Reagent 1 x 20ml Creatinine Standard 1 vial Plate Sealer 2 units -
研究分野
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関連性
Creatinine, or creatine anhydride, is a breakdown product of creatine phosphate in muscle. The loss of water molecule from creatine results in the formation of creatinine. Creatinine is transferred to the kidneys by blood plasma, whereupon it is eliminated from the body by glomerular filtration and partial tubular excretion. Creatinine is usually produced and excreted at a fairly constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function.
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab204537 は 8 報の論文で使用されています。
- Widjaja AA et al. A Neutralizing IL-11 Antibody Improves Renal Function and Increases Lifespan in a Mouse Model of Alport Syndrome. J Am Soc Nephrol 33:718-730 (2022). PubMed: 35140116
- Fan LL et al. Loss of RTN3 phenocopies chronic kidney disease and results in activation of the IGF2-JAK2 pathway in proximal tubular epithelial cells. Exp Mol Med 54:653-661 (2022). PubMed: 35596061
- Gu W et al. Pterostilbene, a Resveratrol Derivative, Improves Ectopic Lipid Deposition in the Kidneys of Mice Induced by a High-Fat Diet. Kidney Blood Press Res 47:514-522 (2022). PubMed: 35717941
- ShamsEldeen AM et al. Combined Systemic Intake of K-ATP Opener (Nicorandil) and Mesenchymal Stem Cells Preconditioned With Nicorandil Alleviates Pancreatic Insufficiency in a Model of Bilateral Renal Ischemia/Reperfusion Injury. Front Physiol 13:934597 (2022). PubMed: 35812319
- Adrianowycz SE et al. Establishment of an RI for the urine ammonia-to-creatinine ratio in dogs. Vet Clin Pathol 50:597-602 (2021). PubMed: 34854107
- Ryu H et al. Urinary creatinine varies with microenvironment and sex in hibernating Greater Horseshoe bats (Rhinolophus ferrumequinum) in Korea. BMC Ecol Evol 21:77 (2021). PubMed: 33947328
- Heidenreich E et al. A Novel UPLC-MS/MS Method Identifies Organ-Specific Dipeptide Profiles. Int J Mol Sci 22:N/A (2021). PubMed: 34576148
- Pierzynowska K et al. Oral Treatment With an Engineered Uricase, ALLN-346, Reduces Hyperuricemia, and Uricosuria in Urate Oxidase-Deficient Mice. Front Med (Lausanne) 7:569215 (2020). PubMed: 33330529