Anti-Creatine Kinase MB 抗体 [CK1] (ab404)

Submit a review Q&A (6)References (2)

Key features and details

  • Mouse monoclonal [CK1] to Creatine Kinase MB
  • Suitable for: ELISA, RIA
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Creatine Kinase MB antibody [CK1]
    Creatine Kinase MB 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [CK1] to Creatine Kinase MB

  • 由来種

    Mouse

  • 特異性

    CK1 recognises human creatine kinase MB isoenzyme. Its affinity for the creatine kinase BB isoenzyme is greater than its affinity for creatine kinase MB isoenzyme. hCK-MB - 100% hCK-BB - 182% hCK-MM - 7.3% Affinity constant: 2x109 l/mol human CK-MB.

  • アプリケーション

    適用あり: ELISA, RIAmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Full length native protein (purified) (Human).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Creatine Kinase MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Creatine Kinase MB reversibly catalyses the transfer of phosphate between ATP and various phosphogens. The creatine kinase isoenzymes play a central role in energy transduction in tissues with large fluctuating energy demands such as skeletal muscle, heart, brain and spermatozoa.

  • 細胞内局在

    Cytoplasmic and Mitochondrial

  • 参照データベース

    • 別名

      • B-CK antibody
      • brain creatine kinase antibody
      • CKBB antibody
      • CKMB antibody
      • CKMM antibody
      • creatine kinase B antibody
      • creatine kinase brain antibody
      • creatine kinase M antibody
      • Creatine kinase MB antibody
      • creatine kinase muscle antibody
      • M-CK antibody
      • muscle creatine kinase antibody
      see all

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献

    ab404 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab404 は 2 報の論文で使用されています。

    • Garay F  et al. Surface plasmon resonance aided electrochemical immunosensor for CK-MB determination in undiluted serum samples. Anal Bioanal Chem 397:1873-81 (2010). PubMed: 20449577
    • Crawford RM  et al. Creatine kinase is physically associated with the cardiac ATP-sensitive K+ channel in vivo. FASEB J 16:102-4 (2002). PubMed: 11729098

    レビューと Q&A

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    1-6 of 6 Abreviews or Q&A

    Question

    I used the mouse monoclonal [CK1] to Creatine Kinase MB (ab404) from Abcam. I have some question to ask you. I would like to know isoelectric point (pI) of this antibody for my applications. I look forward to hearing answer from you. Thank you very much and best regards,

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    Answer

    Thank you for your enquiry. I can confirm the pI range for ab404 Creatine Kinase MB antibody [CK1] is 6.9 to 8.3. Antibodies have ranges of pI rather than points. I hope this information is helpful to you. Should you have any further questions, please do not hesitate to contact us.

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    Question

    I\'d like to know if this product can be used to detect mouse creatine kinase B in immunoistochemistry experiments? (free floating sections of fixed spinal cord. Thank you for your attention

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    Answer

    All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with mouse has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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    Question

    Is this antibody suitable for Immunoprecipitation from TritonX 100 lysates? If yes, what dilutions do you suggest

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    Answer

    This antibody has not been tested in immunoprecipitation, so we can't guarantee that it will work.

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    Question

    Is this antibody suitable for western blotting?

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    Answer

    Dear Gary Please see my answer to your question on ab8364. This is essentially repeated below. This antibody hasn't been tested in Westerns. It is a monoclonal and it works in ELISA and Radioimmunoassays. It is possible that it works in Westerns and I would expect it to work in native gels, however the epitope it recognises may be dependent on tertiary structure and if this is the case it probably wouldn't work in denaturing gels. In general, I believe that ELISA and Radioimmunoassays are performed using native protein. I hope this helps. Regards Michael

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    Question

    Having bought the above product, I need to know a rough dilution value for Western bloting and ELISA. Please suggest a good starting point. Thanks. R. Crawford

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    Answer

    This antibody has not been tested in Western blotting applications, but a good starting dilution for both ELISA and Western would be 1/1000.

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    Question

    Does this antibody recognize mouse CK-MB?

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    Answer

    This antibody has not been tested for cross-reactivity against mouse. However as it was raised in mouse, cross-reactivity against the mouse antigen is unlikely.

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