Application
ELISA
Sample
Human Purified protein (Purified native Human CKMB protein)
Specification
Purified native Human CKMB protein
Type
Sandwich (Capture)
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: MES
Dilution
1/40
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
*Protocol
1. 480 µg carboxyl terminated beads with 2 mM EDC, 5 mM NHS in 500 µl MES buffer r.t 30 min and washing
2. anti-CK-MB from Mouse (ab19603, abcam) 4 µg in 40 µl MES buffer r.t overnight and washing
3. 60 µg antibody conjugated beads with CK-MB antigen (30-AC65, Fitzgerald) r.t 30min in 500 µl whole human serum
4. T-TBS washing 2 times
5. Antigen captured beads + 1.2 µl matrix solution(10 mg/ml sinapinic acid in 50:50:0.1 (v/v/v) acetonitrile, water, trifluoroacetic acid containing 10 fmol/µl cytochrome C as internal standard)
6. Plating and MALDI-TOF analysis
PubMed ID
25036285: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Chang Su Jeon
Verified customer
投稿 Jul 27 2015