Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (Retina)
Permeabilization
No
Specification
Retina
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking Solution
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 488 conjugated goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
No specific staining in the zebrafish retina at 4dpf. Tris-HCl antigen retrieval was used (results were consistent with Na-Citrate). Also heat inactivated goat serum was also tested for blocking resulting in consistent staining.
Abcam response
Thank you for submitting an Abreview. Zebrafish was not a tested species for ab15051 and apparently it does not seem to work well in this species, under these conditions. Thank you for your time, this information will surely be helpful for customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ryan Macdonald
Verified customer
投稿 Jul 30 2013