Anti-CPSF7 抗体 (ab272634)
Key features and details
- Rabbit polyclonal to CPSF7
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CPSF7 antibody -
製品の詳細
Rabbit polyclonal to CPSF7 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human CPSF7 aa 1-150.
Database link: Q8N684 -
ポジティブ・コントロール
- WB: NIH/3T3, NBT-II and RT4 cell lysate. IHC-P: Human esophagus tissue. ICC/IF: A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272634の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates. -
配列類似性
Belongs to the RRM CPSF6/7 family.
Contains 1 RRM (RNA recognition motif) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 79869 Human
- Entrez Gene: 269061 Mouse
- Entrez Gene: 365407 Rat
- Omim: 604979 Human
- SwissProt: Q8N684 Human
- SwissProt: Q8BTV2 Mouse
- SwissProt: Q5XI29 Rat
- Unigene: 718984 Human
see all -
別名
- CFIm59 antibody
- Cleavage and polyadenylation specificity factor 59 kDa subunit antibody
- Cleavage and polyadenylation specificity factor 7 antibody
see all
画像
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All lanes : Anti-CPSF7 antibody (ab272634) at 0.4 µg/ml
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II (Rat bladder carcinoma cell line) cell lysate -
Lane 2 : Anti-CPSF7 antibody (ab272634) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : RT4 (Human urinary bladder cancer cell line) cell lysate -
Paraffin-embedded formalin-fixed human esophagus tissue stained for CPSF7 using ab272634 at 1/50 in immunohistochemical analysis.
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PFA fixed, Triton X-100 permeabilized human cell line A431 labeling CPSF7 (green) using ab272634 at 2 ug/ml in ICC/IF analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272634 は論文での使用が確認できていません。