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  1. Link

    cps1-antibody-ab3682.pdf

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Anti-CPS1 抗体 (ab3682)

  • Datasheet
  • SDS
Reviews (9)Q&A (3)References (19)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-CPS1 antibody (ab3682)
  • Immunocytochemistry/ Immunofluorescence - Anti-CPS1 antibody (ab3682)
  • Western blot - Anti-CPS1 antibody (ab3682)

Key features and details

  • Rabbit polyclonal to CPS1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, African green monkey
  • Isotype: IgG

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human CPS1 protein (ab152296)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-CPS1 antibody
    CPS1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to CPS1
  • 由来種

    Rabbit
  • 特異性

    When the antibody is used at 1/2000 some lower molecular weight bands appear in COS-7 cells, the extent of which is significantly reduced when the antibody is used at 1/10,000.
  • アプリケーション

    適用あり: ICC/IF, WBmore details
  • 種交差性

    交差種: Mouse, Rat, African green monkey
  • 免疫原

    Full length native protein (purified) (Rat).

  • 特記事項

    OMIM reference: 114010 Alternative name: carbamoylphosphate synthetase 1, CAD

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 精製度

    Whole antiserum
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) (ab99697)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human CPS1 protein (ab152296)

アプリケーション

Our Abpromise guarantee covers the use of ab3682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
  • 組織特異性

    Primarily in the liver and small intestine.
  • 関連疾患

    Defects in CPS1 are the cause of carbamoyl phosphate synthetase 1 deficiency (CPS1D) [MIM:237300]. CPS1D is an autosomal recessive disorder of the urea cycle causing hyperammonemia. Clinical features include protein intolerance, intermittent ataxia, seizures, lethargy, developmental delay and mental retardation.
    Note=Genetic variations in CPS1 influence the availability of precursors for nitric oxide (NO) synthesis and play a role in clinical situations where endogenous NO production is critically important, such as neonatal pulmonary hypertension, increased pulmonary artery pressure following surgical repair of congenital heart defects or hepatovenocclusive disease following bone marrow transplantation. Infants with neonatal pulmonary hypertension homozygous for Thr-1406 have lower L-arginine concentrations than neonates homozygous for Asn-1406.
  • 配列類似性

    Contains 2 ATP-grasp domains.
    Contains 1 glutamine amidotransferase type-1 domain.
  • ドメイン

    The type-1 glutamine amidotransferase domain is defective.
  • 細胞内局在

    Mitochondrion.
  • Target information above from: UniProt accession P31327 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 227231 Mouse
    • Entrez Gene: 497840 Rat
    • SwissProt: Q8C196 Mouse
    • SwissProt: P07756 Rat
    • Unigene: 343942 Mouse
    • Unigene: 53968 Rat
    • 別名

      • Carbamoyl phosphate synthase [ammonia] antibody
      • Carbamoyl phosphate synthase [ammonia] mitochondrial antibody
      • Carbamoyl phosphate synthase antibody
      • Carbamoyl phosphate synthetase 1 antibody
      • Carbamoyl phosphate synthetase 1 mitochondrial antibody
      • Carbamoyl phosphate synthetase I antibody
      • Carbamoyl-phosphate synthase [ammonia] antibody
      • Carbamoyl-phosphate synthetase I antibody
      • Carbamoylphosphate synthase antibody
      • Carbamoylphosphate synthetase 1 antibody
      • Carbamoylphosphate synthetase I antibody
      • CPS 1 antibody
      • Cps1 antibody
      • CPSase 1 antibody
      • CPSase I antibody
      • CPSASE1 antibody
      • CPSM_HUMAN antibody
      • mitochondrial antibody
      • MS738 antibody
      see all

    画像

    • Western blot - Anti-CPS1 antibody (ab3682)
      Western blot - Anti-CPS1 antibody (ab3682)

      Lane 1: 5 ul of 2ml lysate cos-7 cells transfected with full length CPS 1. Before IP
      Lane 2: cos-7 cells transfected with full length CPS 1. IP with 5 ul of pre-immune serum
      Lane 3: cos-7 cells transfected with full length CPS 1. IP with 2 ul of polyclonal antibody anti CPS 1 (ab3682)
      Lane 4: cos-7 cells transfected with full length CPS 1. IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682)
      Lane 5: 5 ul of 2ml lysate cos-7 cells transfected with empty vector (pCMV5-tr). Before IP
      Lane 6: cos-7 cells transfected with empty vector (pCMV5-tr). IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682)
      Lane 7: 5 ul of 2ml lysate untransfected cos-7 cells. Before IP
      Lane 8: untransfected cos-7 cells. IP with 5 ul of polyclonal antibody anti CPS 1 (ab3682) Ab3682 can IP CPS1 transiently expressed in COS-7 cells.

      Lane 1: 5 ul of 2ml lysate cos-7 cells transfected with full length CPS 1. Before IP
      Lane 2: cos-7 cells transfected with full length CPS 1. IP

    • Immunocytochemistry/ Immunofluorescence - Anti-CPS1 antibody (ab3682)
      Immunocytochemistry/ Immunofluorescence - Anti-CPS1 antibody (ab3682)

      CPS-1 localisation is consistent with the reticulated pattern of liver mitonchondria.

      Rabbit polyclonal to CPS-1 (ab3682), at 1/5000 on COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV5-CPS 1 plasmid (Corvi, M.M and Berthiaume, L.G. unpublished data).

      Secondary: donkey anti-rabbit (Texas Red).

    • Western blot - Anti-CPS1 antibody (ab3682)
      Western blot - Anti-CPS1 antibody (ab3682)

      Western blot using Rabbit polyclonal to CPS 1 (ab3682). All lanes loaded with 100 ng of purified CPS 1
      Lane 1: ab3682 1/500
      Lane 2: ab3682 1/1000
      Lane 3: ab3682 1/2000 Western blot using Rabbit polyclonal to CPS 1 (ab3682).

      All lanes loaded with 100 ng of purified CPS 1

      Lane 1: ab3682 1/500
      Lane 2: ab3682 1/1000
      Lane 3: ab3682 1/2000

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (19)

    ab3682 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3682 は 19 報の論文で使用されています。

    • Alsaeedi F  et al. Expression of serine/threonine protein kinase SGK1F promotes an hepatoblast state in stem cells directed to differentiate into hepatocytes. PLoS One 14:e0218135 (2019). PubMed: 31242206
    • Tata PR  et al. Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity. Dev Cell 44:679-693.e5 (2018). PubMed: 29587142
    • Wang T  et al. L-leucine stimulates glutamate dehydrogenase activity and glutamate synthesis by regulating mTORC1/SIRT4 pathway in pig liver. Anim Nutr 4:329-337 (2018). PubMed: 30175263
    • Peng WC  et al. Inflammatory Cytokine TNFa Promotes the Long-Term Expansion of Primary Hepatocytes in 3D Culture. Cell 175:1607-1619.e15 (2018). PubMed: 30500539
    • Kang YBA  et al. Metabolic Patterning on a Chip: Towards in vitro Liver Zonation of Primary Rat and Human Hepatocytes. Sci Rep 8:8951 (2018). ICC/IF . PubMed: 29895900
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 12 Abreviews or Q&A

    Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Rat Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 11 2013

    Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Mouse Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 07 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Hepatocyte)
    Specification
    Hepatocyte
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2%
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 12 2013

    Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Liver)
    Loading amount
    50 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 25 2012

    Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Loading amount
    50 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 25 2012

    Question

    Rezepte für Melate-Puffer

    Read More

    Abcam community

    Verified customer

    Asked on Aug 13 2012

    Answer

    Vielen Dank für Ihren Anruf heute Morgen.
    Ich habe mir die Beschreibung des IHC-P Bildes nochmals angeschaut und muss ihnen leider mitteilen, dass ich Ihnen keine genauen Angaben über dem Maleate Puffer machen kann, da dieses Bild und die Beschreibung nicht aus unsrem Labor stammt. Eine Kundin hat einen Abreview als Feedback mi t diesem Bild eingeschickt.
    https://www.abcam.com/index.html?datasheet=3682&tab=abreviews&intabreviewid=14931
    Ich habe allerding Rezepte für Melate-Puffer gefunden:
    http://www.nrims.harvard.edu/protocols/Uranyl_Acetate_Stain_in_Maleate_Buffer.pdf
    http://www.ou.edu/research/electron/bmz5364/maleate.html
    Ich hoffe, diese helfen Ihnen weiter und wünsche Ihnen viel Erfolg mit Ihren Experimenten.

    Read More

    Abcam Scientific Support

    Answered on Aug 13 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Liver)
    Specification
    Liver
    Fixative
    Acetone
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 06 2012

    Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (liver)
    Loading amount
    40 µg
    Specification
    liver
    Treatment
    17.5 mg/kg retrorsine for 24 h
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Junyi Xue

    Verified customer

    投稿 Jul 03 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (liver E18.5)
    Specification
    liver E18.5
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Blocking step
    (agent) for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Read More

    Dr. Asha Seth

    Verified customer

    投稿 Jun 23 2009

    Immunohistochemistry (Frozen sections) abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Liver PO)
    Specification
    Liver PO
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    maleate blocking solution as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Read More

    Dr. Asha Seth

    Verified customer

    投稿 Jun 23 2009

    1-10 of 12 Abreviews or Q&A

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