Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6229] to CPN1 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Human
製品名Anti-CPN1 antibody [EPR6229] - BSA and Azide free
CPN1 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6229] to CPN1 - BSA and Azide free
アプリケーション適用あり: ICC/IF, WBmore details
適用なし: Flow Cyt,IHC-P or IP
Synthetic peptide within Human CPN1 aa 100-200. The exact sequence is proprietary.
ab248031 is the carrier-free version of ab124874 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248031 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
Concentration information loading...
Our Abpromise guarantee covers the use of ab248031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 55, 48, 27 kDa (predicted molecular weight: 52 kDa).
Note: The 48-kDa form is generated by proteolytic cleavage at the C-terminus. This subunit is cleaved into a peptide.
機能Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.
組織特異性Synthesized in the liver and secreted in plasma.
関連疾患Defects in CPN1 are the cause of carboxypeptidase N deficiency (CPND) [MIM:212070]. Patients affected present some combination of angioedema or chronic urticaria, as well as hay fever or astma, and have also slightly depressed serum carboxy peptidase N, suggestive of autosomal recessive inheritance of this disorder.
配列類似性Belongs to the peptidase M14 family.
細胞内局在Secreted > extracellular space.
- Information by UniProt
- ACBP antibody
- Anaphylatoxin inactivator antibody
- Arginine carboxypeptidase antibody
All lanes : Anti-CPN1 antibody [EPR6229] (ab124874) at 1/1000 dilution
Lane 1 : Human plasma lysate
Lane 2 : HepG2 lysate
Lane 3 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Additional bands at: 27 kDa (possible cleavage fragment), 48 kDa (possible cleavage fragment)
This data was developed using ab124874, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.