Application
Western blot
Sample
Human Cell lysate - whole cell (Hela cells)
Gel Running Conditions
Reduced Denaturing (5-20% gradient)
Loading amount
20 µg
Specification
Hela cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Peroxidase AffiniPure F(ab')2 Fragment Donkey Anti
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
none
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: 250, 150, 120, 60, 55, 35 kDa
Positive control
none
Additional data
Abcam response
This is the first human western blot data we are aware of for this antibody, which was raised against a peptide from mouse CPEB3. We recommend including a positive control of murine cell or tissue lysate if possible when testing human samples. In acccordance with our Abtrial progam (www.abacm.acom/abtrial), the reviewer will receive credit towards a future purchase.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 21 2015