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Anti-CP110 抗体 (ab99337)

Submit a review Q&A (4)References (2)
Western blot - Anti-CP110 antibody (ab99337)
  • Immunoprecipitation - Anti-CP110 antibody (ab99337)

Key features and details

  • Rabbit polyclonal to CP110
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-CP110 antibody
    CP110 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to CP110

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla

  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids within residues 575-625 of Human CP110 (CAH18111.1).

  • ポジティブ･コントロール

    • HeLa and 293T whole cell lysates

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab99337の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/2000 - 1/10000. Predicted molecular weight: 113 kDa.
IP
Use at 2-5 µg/mg of lysate.
特記事項
WB
1/2000 - 1/10000. Predicted molecular weight: 113 kDa.
IP
Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能

    Necessary for centrosome duplication at different stages of procentriole formation. Collaborates with CEP97, being involved in the suppression of a cilia assembly program. Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2.

  • 組織特異性

    Highly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.

  • 翻訳後修飾

    Phosphorylated by CDKs.
    Ubiquitinated by the SCF(Cyclin F) during G2 phase, leading to its degradation by the proteasome and preventing centrosome reduplication.

  • 細胞内局在

    Cytoplasm > cytoskeleton > centrosome > centriole. Recruited early and then associates with the growing distal tips.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 110 kDa centrosomal protein antibody
      • CCP110 antibody
      • Centriolar coiled coil protein 110kDa antibody
      • Centrosomal protein 110 kDa antibody
      • Centrosomal protein CP110 antibody
      • Centrosomal protein of 110 kDa antibody
      • Cep110 antibody
      • CP110 antibody
      • CP110_HUMAN antibody
      • DKFZp781G1416 antibody
      • FAN antibody
      • KIAA0419 antibody
      see all

    画像

    • Western blot - Anti-CP110 antibody (ab99337)
      Western blot - Anti-CP110 antibody (ab99337)
      All lanes : Anti-CP110 antibody (ab99337) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 113 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-CP110 antibody (ab99337)
      Immunoprecipitation - Anti-CP110 antibody (ab99337)
      ab99337 at 1µg/ml detecting CP110 in HeLa whole cell lysate by WB following IP.

      Lane 1: ab99337 IP at 3µg/mg of lysate
      Lane 2: IP with an antibody which recognizes an downstream epitope of CP110
      Lane 3: control IgG.

      In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
      Detection: Chemiluminescence with an exposure time of 30 seconds.

    参考文献 (2)

    ab99337 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab99337 は 2 報の論文で使用されています。

    • Rowlison T  et al. Deciphering the mechanisms involving cenexin, ninein and centriolin in sperm maturation during epididymal transit in the domestic cat. Reprod Domest Anim 52 Suppl 2:193-196 (2017). PubMed: 27807895
    • Hori A  et al. Centriolar satellite- and hMsd1/SSX2IP-dependent microtubule anchoring is critical for centriole assembly. Mol Biol Cell 26:2005-19 (2015). ICC/IF ; Human . PubMed: 25833712

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Question

    Dear Technical Team,

    Hope this e-mail finds you well! Please find below a complaint:

    Request you to kindly look into it & advise us accordingly to do further needful on it.

    Looking forward towards your valued reply/ comments on it.

    Thanking you in advance.

    With Best Regards,

    This is to bring to your attention that Cat nos. are as

    follows:

    1. ab11257 Rabbit polyclonal to Centrin 1 and

    2. ab 99337 Rabbit polyclonal to CP110

    We have received these two antibodies and these have been checked by immunoflouresence. These antibodies are not working as they are not giving the required signals. We have tried all the protocols given in various references including the one provided by ABCAM.This includes trying different fixatives, detergents for permeabilization, different blocking agents and various dilutions of the antibodies. We have repeated it several times but to no avail.It has been a complete wastage of my time and resourses. My work is suffering due to this.

    There is no error at my side and no excuse will be entertained in this regard. I just want the antibody to be replaced asap, either with the same cat no or some other catalog no and both should work for sure .

    Please don't waste our time by multiple formalities, and do the needful asap.

    With thanks,

    Read More
    Answer

    Thank you for your enquiry regarding our two products. I am very sorry to hear that you are having problems with this antibody.

    As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

    Tested and Guaranted Applications:

    ab11257 - WB, ICC/IF

    ab99337 - WB and IP - this has not been tested in immunostaining

    Could you please provide some details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

    - Batch/Order numbers,

    - Species and type of the sample,

    - Cells or tissue section used,

    - Fixation method

    Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

    Read More

    Question

    Please see below reply from this customer regarding Anti-CP110 antibody (ab99337) & also the attached immunoblot.

    This is with reference to replacement of two ABCAM antibodies, centrin 1 ( ab

    11257) and CP110(ab 99337).

    I am sending an immunoblot of CP110.As you will find the antibody is not working for IB either. It is giving many non specific bands.

    So kindly replace it and the Centrin antibody.

    Read More
    Answer

    Thank you for your response.

    I am not sure what IB represents but assume the customer would mean either IP (Immunoprecipitation) or WB (Western blot) application? Is that correct?

    I regret to inform you that I am not convinced that this antibody (ab99337) is faulty. However, I appreciate your long-term relationship with this customer and try to help you to close this case soon.

    Here are a few comments I would like to make and share:

    Multiple bands could be due to several factors (blocking, protein degradation, secondary antibody). Since the non-specific bands are lower range than the expected band size for CP110, it may well be that the bands are degradation product.

    Unfortunately, I have limited information about the experiments but in future in similar complaint the following details would be advised to provide in order to get sufficient technical support

    What Lane 1 and 2 represent? Identical samples? Or different preparation?
    How was the blocking applied (blocking agent, time and temperature)?
    How much protein was loaded?
    Has "No primary" (only secondary) antibody control was used to rule out any non-specific signal due to the secondary antibody?



    Please be advised that there are two isoforms (O43303-1 and O43303-2) which have been described in the literature and it would be advisable to check which one the customer’s samples express. Your customer could find some further information about this target at this site:

    http://www.uniprot.org/uniprot/O43303

    Furthermore, CCP110 has different subunits and the CCP110 protein can interact with other proteins such CALM1, CETN2, CEP76, CEP97, NEURL4 and CCNF. For this reason, it is essential to know if the experiments were carried out under naturing or denaturing/and reducing conditions. This information is also missing in the last e-mail and would be very important to know.

    Currently, we have two alternative antibodies against CP110 ab99338 and ab91415 and can offer one free of charge replacement vial. Could you please discuss the content of this e-mail with your customer and you let me know which product he wishes to test.

    Thank you for your continued cooperation.

    Read More

    Question

    Thank you for your below reply.

    We have been able to convince this customer for further informations on this complaint, please see the attached technical form and the IF images sent by this customer.

    Kindly advise.

    Looking forward towards your valued response on it.

    With Best Regards,

    Read More
    Answer

    Thank you for your response.

    Unfortunately, the two image files can't be opened so I am unable to take a look at the signal.

    Tested and Guaranted Applications:

    ab11257 - WB, ICC/IF

    ab99337 - WB and IP - this has not been tested in immunostaining.

    Since ab99337 has not been tested in immunostaining, I am very sorry but our Abpromise does not apply for this antibody.

    Regarding ab11257, the protocol looks fine though it would be interesting to know if the cultured HeLa cells are from the exponential phase of growth or from a confluent culture. No information is provided. Furthermore, blocking non-specific bindings with 10% BSA may be a bit too strong with cultured cells.

    I can offer a new free of charge vial of 11257 but not for the other product ab99337 which has not been tested for ICC/IF yet.

    Could you please discuss our policy with the customer to make sure he understands for which product we can offer the Abpromise.

    I look forward to hearing from you soon.

    Read More

    Question

    Hi, I am a postdoc in Department of Genetics. I ordered an anti-CP110 antibody ab99337 from you two weeks ago, and while I tested it for western blotting, I couldn't see specific bands at the right size as your pictures show on the website. Of course I had a positive control using the same samples and a different antibody and I got the bands as expected. I was wondering since you have another product ab99338, if I could swap 99337 for it.
    Thanks very much!

    Read More
    Answer

    Thank you for contacting us and reporting the problems encountered when using anti-CP110 antibody (ab99337).

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    In order to understand the situation more fully could you please fill out the questionnaire I have attached to this email. This should not take more than 5-10 minutesand theinformation provided willallowme to investigate this case internally and initiate additional testing where necessary. If you would be able to share images ofthe blots youhave obtained with ab99337 and thatof theother antibodyused that would be very helpful.

    If the product was purchased in the last six monthsand is being used according to our Abpromise, we would be happy to replace or refund the antibody.

    I look forward to receiving your reply.

    Read More

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