Anti-Coxsackie Adenovirus Receptor/hCAR 抗体 [EPR23305-44] (ab272711)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23305-44] to Coxsackie Adenovirus Receptor/hCAR
- Suitable for: Flow Cyt, WB, IP, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Coxsackie Adenovirus Receptor/hCAR antibody [EPR23305-44]
Coxsackie Adenovirus Receptor/hCAR 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23305-44] to Coxsackie Adenovirus Receptor/hCAR -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt, WB, IP, ICC/IFmore details
適用なし: IHC-P -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: LoVo, HepG2, HaCaT, BxPC-3, Human brain and Human liver, Human heart lysates. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells. IP: HepG2 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23305-44 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272711の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
1/500.
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WB |
1/1000. Predicted molecular weight: 40 kDa.
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IP |
1/30.
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ICC/IF |
1/50.
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特記事項 |
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Flow Cyt
1/500. |
WB
1/1000. Predicted molecular weight: 40 kDa. |
IP
1/30. |
ICC/IF
1/50. |
ターゲット情報
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機能
Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN. -
組織特異性
Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy. -
配列類似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
ドメイン
The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
The PDZ-binding motif mediates interaction with MPDZ and BAIAP1. -
翻訳後修飾
N-glycosylated.
Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane. -
細胞内局在
Secreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. - Information by UniProt
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参照データベース
- Entrez Gene: 1525 Human
- Omim: 602621 Human
- SwissProt: P78310 Human
- Unigene: 634837 Human
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別名
- 46 kD coxsackievirus and adenovirus receptor CAR protein antibody
- 46 kD coxsackievirus and adenovirus receptor protein antibody
- CAR antibody
see all
画像
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling Coxsackie Adenovirus Receptor/hCAR with ab272711 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in HepG2 cell line. Negative control: K562 (PMID: 30450519) ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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All lanes : Anti-Coxsackie Adenovirus Receptor/hCAR antibody [EPR23305-44] (ab272711) at 1/1000 dilution
Lane 1 : LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : HaCaT (human skin keratinocyte) whole cell lysate
Lane 5 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 6 : BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 35-50 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature, differently mobilized bands of coxsackie adenovirus receptor are attributed to different levels of glycosylation and the presence of isoforms. (PMID: 28074864, 11734628)
Negative control: PC-3 (PMID: 28074864) and K-562 (PMID: 30450519).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes
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All lanes : Anti-Coxsackie Adenovirus Receptor/hCAR antibody [EPR23305-44] (ab272711) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Human heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 40 kDa
Observed band size: 35,45 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 28074864, 11734628)
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 125 seconds
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Coxsackie Adenovirus Receptor/hCAR was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab272711 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272711 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: ab272711 IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272711 in HepG2 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 102 seconds
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Flow cytometric analysis of K-562 (Human chronic myelogenous leukemia lymphoblast, Left) / HepG2 (Human hepatocellular carcinoma epithelial cell, Right) cells labelling Coxsackie Adenovirus Receptor/hCAR with ab272711 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: K-562 (PMID: 30450519)Gated on viable cells.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab272711 は 1 報の論文で使用されています。
- Fu Y & Xiong S Exosomes mediate Coxsackievirus B3 transmission and expand the viral tropism. PLoS Pathog 19:e1011090 (2023). PubMed: 36634130