Anti-Coxsackie Adenovirus Receptor/hCAR 抗体 (ab100811)
Key features and details
- Rabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Coxsackie Adenovirus Receptor/hCAR antibody
Coxsackie Adenovirus Receptor/hCAR 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
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免疫原
Synthetic peptide corresponding to Human Coxsackie Adenovirus Receptor/hCAR aa 300-400. NP_001329.1
Database link: P78310 -
ポジティブ･コントロール
- HeLa whole cell lysate (ab150035)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab100811の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 40 kDa.
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| IP | (1) |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 40 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN. -
組織特異性
Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy. -
配列類似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
ドメイン
The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
The PDZ-binding motif mediates interaction with MPDZ and BAIAP1. -
翻訳後修飾
N-glycosylated.
Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane. -
細胞内局在
Secreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. - Information by UniProt
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参照データベース
- Entrez Gene: 281733 Cow
- Entrez Gene: 403772 Dog
- Entrez Gene: 1525 Human
- Entrez Gene: 13052 Mouse
- Entrez Gene: 100174624 Orangutan
- Entrez Gene: 397333 Pig
- Entrez Gene: 100302411 Rabbit
- Entrez Gene: 89843 Rat
see all -
別名
- 46 kD coxsackievirus and adenovirus receptor CAR protein antibody
- 46 kD coxsackievirus and adenovirus receptor protein antibody
- CAR antibody
see all
画像
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Western blot - Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab100811)All lanes : Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab100811) at 0.1 µg/ml
Lane 1 : HeLa Whole Cell Lysate at 50 µg
Lane 2 : HeLa Whole Cell Lysate at 15 µg
Lane 3 : HeLa Whole Cell Lysate at 5 µg
Predicted band size: 40 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab100811)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Coxsackie Adenovirus Receptor/hCAR with ab100811 at 1/1000 (1µg/ml). Detection: Peroxidase Substrate.
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Immunoprecipitation - Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab100811)ab100811 at 0.4 µg/ml staining Coxsackie Adenovirus Receptor/hCAR in HeLa cell lysate immunoprecipitated using ab100811 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 30 seconds
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab100811 は 4 報の論文で使用されています。
- Huang Y et al. A SIRPa-Fc fusion protein enhances the antitumor effect of oncolytic adenovirus against ovarian cancer. Mol Oncol 14:657-668 (2020). PubMed: 31899582
- Weider T et al. Upregulation of HLA Class I and Antiviral Tissue Responses in Hashimoto's Thyroiditis. Thyroid 30:432-442 (2020). PubMed: 31910110
- Lalwani A et al. ß Cell Hypoxia-Inducible Factor-1a Is Required for the Prevention of Type 1 Diabetes. Cell Rep 27:2370-2384.e6 (2019). PubMed: 31116982
- Ulasov IV et al. Tamoxifen improves cytopathic effect of oncolytic adenovirus in primary glioblastoma cells mediated through autophagy. Oncotarget 6:3977-87 (2015). WB . PubMed: 25738357
