Anti-COX2 / Cyclooxygenase 2 抗体 (ab88522)
Key features and details
- Mouse polyclonal to COX2 / Cyclooxygenase 2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-COX2 / Cyclooxygenase 2 antibody
COX2 / Cyclooxygenase 2 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to COX2 / Cyclooxygenase 2 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human COX2/ Cyclooxygenase 2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- Jurkat cell lysate and COX2/Cyclooxygenase 2 transfected 293T cell lysate, HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88522の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
パスウェイ
Lipid metabolism; prostaglandin biosynthesis. -
配列類似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
翻訳後修飾
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
細胞内局在
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5743 Human
- Omim: 600262 Human
- SwissProt: P35354 Human
- Unigene: 196384 Human
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別名
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
画像
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Anti-COX2 / Cyclooxygenase 2 antibody (ab88522) at 1 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 69 kDa -
All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab88522) at 1 µg/ml
Lane 1 : COX2 / Cyclooxygenase 2 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 69 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted? -
ab88522 at 10 µg/ml staining COX2 / Cyclooxygenase 2 in paraformaldehyde fixed, permeabilised HeLa cells, by immunofluorescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab88522 は 1 報の論文で使用されています。
- Zhang Z MiR-124-3p Suppresses Prostatic Carcinoma by Targeting PTGS2 Through the AKT/NF-?B Pathway. Mol Biotechnol 63:621-630 (2021). PubMed: 33893997