Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (adult brain, hippocampus)
Specification
adult brain, hippocampus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid pH6 10mM
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/400
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/Azide
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/300
Additional data
Additional Notes
My standard stABCpx-DAB procedure shows (see picture) clear cytoplasmic immunostaining in a subset of neurones in the dentate gyrus of a sagittal section of whole rat brain.
In other areas of this section, there are, additionally, small cells and their processes that are positive: they may be inter-neurones or microglia: it would be neccessary to carry out double-immunostaining to confirm this.
In other areas of this section, there are, additionally, small cells and their processes that are positive: they may be inter-neurones or microglia: it would be neccessary to carry out double-immunostaining to confirm this.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Feb 07 2008