Anti-COX2 / Cyclooxygenase 2 抗体 (ab15191)
Key features and details
- Rabbit polyclonal to COX2 / Cyclooxygenase 2
- Suitable for: WB, Sandwich ELISA, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-COX2 / Cyclooxygenase 2 antibody
COX2 / Cyclooxygenase 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to COX2 / Cyclooxygenase 2 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WB, Sandwich ELISA, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Syrian hamster -
免疫原
Synthetic peptide within Rat COX2/ Cyclooxygenase 2 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P35355 -
特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
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- Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15191の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (19) |
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
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Sandwich ELISA |
Use a concentration of 0.5 µg/ml.
For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab90345 as Capture. |
|
IHC-P | (8) |
1/100.
|
特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa. |
Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab90345 as Capture. |
IHC-P
1/100. |
ターゲット情報
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機能
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
パスウェイ
Lipid metabolism; prostaglandin biosynthesis. -
配列類似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
翻訳後修飾
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
細胞内局在
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5743 Human
- Entrez Gene: 19225 Mouse
- Entrez Gene: 29527 Rat
- Omim: 600262 Human
- SwissProt: P35354 Human
- SwissProt: Q05769 Mouse
- SwissProt: P35355 Rat
- Unigene: 196384 Human
see all -
別名
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
画像
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All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab15191) at 1 µg/ml
Lane 1 : Recombinant human COX2 / Cyclooxygenase 2 protein (Active) (ab58868) at 0.1 µg
Lane 2 : Recombinant human COX2 / Cyclooxygenase 2 protein (Active) (ab58868) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Exposure time: 10 seconds -
ab15191 staining mouse brain sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 1% BSA for 10 minutes at RT. The primary antibody was diluted 1/2000 and incubated with the sample for 2 hours. A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary. Sections were preblocked in an Avidin-Biotin kit to mask endogenous biotin
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ab15191 staining COX2 / Cyclooxygenase in human breast carcinoma by Immunohistochemistry (FFPE-sections).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (394)
ab15191 は 394 報の論文で使用されています。
- Lee H et al. Protective Effect of Gamma Aminobutyric Acid against Aggravation of Renal Injury Caused by High Salt Intake in Cisplatin-Induced Nephrotoxicity. Int J Mol Sci 23:N/A (2022). PubMed: 35008928
- Liu TT et al. Efficacy profile of noninvasive vagus nerve stimulation on cortical spreading depression susceptibility and the tissue response in a rat model. J Headache Pain 23:12 (2022). PubMed: 35062860
- Qiao W et al. Divalent metal cations stimulate skeleton interoception for new bone formation in mouse injury models. Nat Commun 13:535 (2022). PubMed: 35087048
- De Matteis R et al. Aspirin activates resolution pathways to reprogram T cell and macrophage responses in colitis-associated colorectal cancer. Sci Adv 8:eabl5420 (2022). PubMed: 35108049
- Du X et al. Circular RNA hsa_circ_0083756 promotes intervertebral disc degeneration by sponging miR-558 and regulating TREM1 expression. Cell Prolif 55:e13205 (2022). PubMed: 35187741