Anti-COX2 / Cyclooxygenase 2 抗体 (ab15191)
製品の概要
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製品名
Anti-COX2 / Cyclooxygenase 2 antibody
COX2 / Cyclooxygenase 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to COX2 / Cyclooxygenase 2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Fr, ICC/IF, Sandwich ELISA, IP, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human, Syrian hamster -
免疫原
Synthetic peptide within Rat COX2/ Cyclooxygenase 2 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P35355 -
ポジティブ･コントロール
- IHC-P: Mouse brain, Rat hippocampus, and Human breast carcinoma tissues; IHC-Fr: Mouse aorta ateriosclerotic plaque, Mouse liver, and Human colorectal tumor tissues; ICC/IF: HepG2 cells;
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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アプリケーション
Our Abpromise guarantee covers the use of ab15191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | 1/100. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 20660112 | |
| Sandwich ELISA | Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab90345 as Capture. |
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| IP | Use at an assay dependent concentration. | |
| IHC-P | 1/100. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 69 kDa. |
ターゲット情報
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機能
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
パスウェイ
Lipid metabolism; prostaglandin biosynthesis. -
配列類似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
翻訳後修飾
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
細胞内局在
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5743 Human
- Entrez Gene: 19225 Mouse
- Entrez Gene: 29527 Rat
- Omim: 600262 Human
- SwissProt: P35354 Human
- SwissProt: Q05769 Mouse
- SwissProt: P35355 Rat
- Unigene: 196384 Human
see all -
別名
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
画像
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab15191) at 1 µg/ml
Lane 1 : Recombinant human COX2 / Cyclooxygenase 2 protein (Active) (ab58868) at 0.1 µg
Lane 2 : Recombinant human COX2 / Cyclooxygenase 2 protein (Active) (ab58868) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)ab15191 staining COX2 in HepG2 cells treated with sinomenine hydrochloride (ab141190), by ICC/IF. Decrease of COX2 expression correlates with increased concentration of sinomenine hydrochloride, as described in literature.
The cells were incubated at 37°C for 48 hours in media containing different concentrations of ab141190 (sinomenine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab15191 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)This image is courtesy of an Abreview submitted by Mr Carl Hobbsab15191 staining mouse brain sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 1% BSA for 10 minutes at RT. The primary antibody was diluted 1/2000 and incubated with the sample for 2 hours. A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary. Sections were preblocked in an Avidin-Biotin kit to mask endogenous biotin
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Sandwich ELISA - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)Standard Curve for COX2 / Cyclooxygenase 2 (Analyte: ab58868) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS66] to COX2 / Cyclooxygenase 2 (ab90345) at 1ug/ml and Detector Antibody Rabbit polyclonal to COX2 / Cyclooxygenase 2 (ab15191) at 0.5ug/ml -
Immunohistochemistry (Frozen sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)Image is courtesy of an anonymous Abreviewab15191 staining COX2 in Human Colorectal Tumor tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 5% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/500 in PBS + 0.3% Triton + 0.1% BSA) for 2 hours at 24°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)This image is courtesy of an Abreview submitted by Mr Carl Hobbsab15191 staining adult rat hippocampus tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval (in citric acid, pH6, 10mM) prior to blocking in 1% BSA for 10 minutes at RT. The primary anitbody was diluted 1/400 and incubated with the sample for 2 hours. A biotinylated goat anti-rabbit antibody, diluted 1/300 was used as the secondary.
Clear cytoplasmic staining in a subset of neurones in the dentate gyrus of a sagittal section of whole rat brain can be seen. In other areas of this section, there are, additionally, small cells and their processes that are positive: they may be inter-neurones or microglia: it would be neccessary to carry out double-immunostaining to confirm this.
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Immunohistochemistry (Frozen sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)This image is courtesy of an Abreview submitted by Miss Silke Vorwaldab15191 staining tissue sections of arteriosclerotic plaque in mouse aorta by IHC-Fr. Sections were acetone fixed and blocked in 1% serum for 10 minutes at 20°C prior to incubation with the primary antibody (diluted 1/500) for 1 hour at 20°C. A biotinylated donkey anti-rabbit antibody (diluted 1/500) was used as the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)ab15191 staining COX2 / Cyclooxygenase in human breast carcinoma by Immunohistochemistry (FFPE-sections).
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Immunohistochemistry (Frozen sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)This image is courtesy of an anonymous Abreviewab15191 staining COX2 / Cyclooxygenase 2 in Mouse liver tissue sections by IHC-Fr.
Cells were fixed with acetone and blocked with 10% Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 0.1% Triton x-100) for 13 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal(1/1000) was used as the secondary antibody.
プロトコール
参考文献
This product has been referenced in:
- Soliman NA et al. The possible ameliorative effect of simvastatin versus sulfasalazine on acetic acid induced ulcerative colitis in adult rats. Chem Biol Interact 298:57-65 (2019). Read more (PubMed: 30408459) »
- Liu LM et al. Edaravone acts as a potential therapeutic drug against pentylenetetrazole-induced epilepsy in male albino rats by downregulating cyclooxygenase-II. Brain Behav 9:e01156 (2019). Read more (PubMed: 30506635) »
