Anti-COX2 / Cyclooxygenase 2 抗体 (ab15191)

ab15191 staining mouse brain sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 1% BSA for 10 minutes at RT.  The primary antibody was diluted 1/2000 and incubated with the sample for 2 hours.  A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary. Sections were preblocked in an Avidin-Biotin kit to mask endogenous biotin

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ab15191 staining COX2 in Human Colorectal Tumor tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 5% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/500 in PBS + 0.3% Triton + 0.1% BSA) for 2 hours at 24°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

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ab15191 staining adult rat hippocampus tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval (in citric acid, pH6, 10mM) prior to blocking in 1% BSA for 10 minutes at RT.  The primary anitbody was diluted 1/400 and incubated with the sample for 2 hours.  A biotinylated goat anti-rabbit antibody, diluted 1/300 was used as the secondary.

Clear cytoplasmic staining in a subset of neurones in the dentate gyrus of a sagittal section of whole rat brain can be seen.  In other areas of this section, there are, additionally, small cells and their processes that are positive: they may be inter-neurones or microglia: it would be neccessary to carry out double-immunostaining to confirm this.

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ab15191 staining tissue sections of arteriosclerotic plaque in mouse aorta by IHC-Fr.  Sections were acetone fixed and blocked in 1% serum for 10 minutes at 20°C prior to incubation with the primary antibody (diluted 1/500) for 1 hour at 20°C.  A biotinylated donkey anti-rabbit antibody (diluted 1/500) was used as the secondary.

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ab15191 staining COX2 / Cyclooxygenase in human breast carcinoma by Immunohistochemistry (FFPE-sections).

ab15191 staining COX2 / Cyclooxygenase 2 in Mouse liver tissue sections by IHC-Fr.

Cells were fixed with acetone and blocked with 10% Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 0.1% Triton x-100) for 13 hour at 4°C. An Alexa Fluor^®488-conjugated Goat anti-rabbit IgG polyclonal(1/1000) was used as the secondary antibody.

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