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    cox2--cyclooxygenase-2-antibody-ab15191.pdf

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Anti-COX2 / Cyclooxygenase 2 抗体 (ab15191)

  • Datasheet
  • SDS
Reviews (36)Q&A (20)References (321)

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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)
  • Sandwich ELISA - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)

Key features and details

  • Rabbit polyclonal to COX2 / Cyclooxygenase 2
  • Suitable for: WB, Sandwich ELISA, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (ab179800)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-COX2 / Cyclooxygenase 2 antibody
    COX2 / Cyclooxygenase 2 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to COX2 / Cyclooxygenase 2
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, Sandwich ELISA, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, Syrian hamster
  • 免疫原

    Synthetic peptide within Rat COX2/ Cyclooxygenase 2 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P35355

  • 特記事項

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Prostaglandins
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human COX2 / Cyclooxygenase 2 protein (ab198646)
  • Related Products

    • Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
    • Anti-COX5A antibody [6E9B12D5] (ab110262)
    • Anti-COX5B antibody [16H12H9] (ab110263)
    • Anti-COX6A1 antibody [14A3AD2BH4] (ab110265)
    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Sinomenine hydrochloride, Morphinan analog (ab141190)
    • Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab15191の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (19)
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
Sandwich ELISA
Use a concentration of 0.5 µg/ml.

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab90345 as Capture.

IHC-P (7)
1/100.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
Sandwich ELISA
Use a concentration of 0.5 µg/ml.

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab90345 as Capture.

IHC-P
1/100.

ターゲット情報

  • 機能

    Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • パスウェイ

    Lipid metabolism; prostaglandin biosynthesis.
  • 配列類似性

    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • 翻訳後修飾

    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • 細胞内局在

    Microsome membrane. Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession P35354 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 5743 Human
    • Entrez Gene: 19225 Mouse
    • Entrez Gene: 29527 Rat
    • Omim: 600262 Human
    • SwissProt: P35354 Human
    • SwissProt: Q05769 Mouse
    • SwissProt: P35355 Rat
    • Unigene: 196384 Human
    • Unigene: 292547 Mouse
    • Unigene: 44369 Rat
    see all
  • 別名

    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC 1.14.99.1 antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

画像

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)
    All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab15191) at 1 µg/ml

    Lane 1 : Recombinant human COX2 / Cyclooxygenase 2 protein (Active) (ab58868) at 0.1 µg
    Lane 2 : Recombinant human COX2 / Cyclooxygenase 2 protein (Active) (ab58868) at 0.01 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 69 kDa


    Exposure time: 10 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)This image is courtesy of an Abreview submitted by Mr Carl Hobbs

    ab15191 staining mouse brain sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 1% BSA for 10 minutes at RT.  The primary antibody was diluted 1/2000 and incubated with the sample for 2 hours.  A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary. Sections were preblocked in an Avidin-Biotin kit to mask endogenous biotin

    See Abreview

  • Sandwich ELISA - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)
    Sandwich ELISA - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)

    Standard Curve for COX2 / Cyclooxygenase 2 (Analyte: ab58868) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS66] to COX2 / Cyclooxygenase 2 (ab90345) at 1ug/ml and Detector Antibody Rabbit polyclonal to COX2 / Cyclooxygenase 2 (ab15191) at 0.5ug/ml

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)

    ab15191 staining COX2 / Cyclooxygenase in human breast carcinoma by Immunohistochemistry (FFPE-sections).

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Protocol Booklet

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (321)

ab15191 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab15191 は 321 報の論文で使用されています。

  • Xu K  et al. SIRT3 ameliorates osteoarthritis via regulating chondrocyte autophagy and apoptosis through the PI3K/Akt/mTOR pathway. Int J Biol Macromol 175:351-360 (2021). PubMed: 33556400
  • Li T  et al. TRPV1 feed-forward sensitisation depends on COX2 upregulation in primary sensory neurons. Sci Rep 11:3514 (2021). PubMed: 33568699
  • Shi FJ  et al. Is Iba-1 protein expression a sensitive marker for microglia activation in experimental diabetic retinopathy? Int J Ophthalmol 14:200-208 (2021). PubMed: 33614447
  • Bakalarz D  et al. Organic carbon monoxide prodrug, BW-CO-111, in protection against chemically-induced gastric mucosal damage. Acta Pharm Sin B 11:456-475 (2021). PubMed: 33643824
  • Sun Q  et al. Parathyroid hormone attenuates osteoarthritis pain by remodeling subchondral bone in mice. Elife 10:N/A (2021). PubMed: 33646122
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 56 Abreviews or Q&A

Western blot abreview for Anti-COX2 / Cyclooxygenase 2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (colon)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris)
Loading amount
15 µg
Specification
colon
Blocking step
Commercial blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Nov 01 2021

Western blot abreview for Anti-COX2 / Cyclooxygenase 2 antibody

Average
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell (BRAIN)
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20% gradient)
Loading amount
30 µg
Specification
BRAIN
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Read More

Abcam user community

Verified customer

投稿 Oct 27 2019

Western blot abreview for Anti-COX2 / Cyclooxygenase 2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (skin)
Gel Running Conditions
Reduced Denaturing (10% Acrylamide gel)
Loading amount
20 µg
Treatment
UV B 100mJ/cm2
Specification
skin
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

MR. Jeong-Yong Park

Verified customer

投稿 Mar 15 2019

Western blot abreview for Anti-COX2 / Cyclooxygenase 2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Liver)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
40 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 May 24 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-COX2 / Cyclooxygenase 2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1(Ventana EDTA)
Permeabilization
No
Specification
Colon
Fixative
10% neutral formalin
Read More

Abcam user community

Verified customer

投稿 Oct 12 2017

Western blot abreview for Anti-COX2 / Cyclooxygenase 2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (human brain)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
20 µg
Treatment
huntington's disease
Specification
human brain
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 May 16 2017

Western blot abreview for Anti-COX2 / Cyclooxygenase 2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse brain (striatum) lysate)
Gel Running Conditions
Reduced Denaturing (4-20% TGX stain free (Tris gly))
Loading amount
20 µg
Specification
Mouse brain (striatum) lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Ms. Misha Riley

Verified customer

投稿 May 05 2017

Western blot abreview for Anti-COX2 / Cyclooxygenase 2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Abdominal endometriotic lesion)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
15 µg
Specification
Abdominal endometriotic lesion
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

投稿 Mar 21 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-COX2 / Cyclooxygenase 2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (peritonei tumor)
Permeabilization
Yes - 0.3% Triton
Specification
peritonei tumor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 07 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-COX2 / Cyclooxygenase 2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LS174T)
Permeabilization
Yes - 0.3% TritonX -100
Specification
LS174T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 01 2017

1-10 of 56 Abreviews or Q&A

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