Key features and details
- Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
製品名Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4]
COX1 / Cyclooxygenase 1 一次抗体 製品一覧
製品の詳細Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1
アプリケーション適用あり: Flow Cytmore details
種交差性交差種: Mouse, Human
Other Immunogen Type corresponding to COX1/ Cyclooxygenase 1. A preparation of intracellular proteins of the human cell line HL60.
- WB: Huh-7 whole cell lysate. COX1 protein standard prepared from ram seminal vesicles. Flow Cytometry: NIH/3T3 cells. IHC-P: Human anal cancer tissue. Mouse kidney tissue. IHC-Fr: Human breast and breast tumor tissue. IP: Huh-7 whole cell lysate.
特記事項Please note that we have received reports from customers working with this antibody that they obtain two stong bands in Western blot. A single band at around 72kDa is expected. One customer reported that when tested with three different human cell lines two strong bands are obtained, one between 40 - 50kDa and other other between 62 - 83kDa. It is possible that a degraded form of the enzyme is detected or the bands may correspond to different glycosylation states.
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
バッファーPreservative: 0.02% Sodium azide
Concentration information loading...
精製度Immunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
Our Abpromise guarantee covers the use of ab695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
機能May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
パスウェイLipid metabolism; prostaglandin biosynthesis.
配列類似性Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain.
細胞内局在Microsome membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- COX 1 antibody
- COX 3 antibody
- COX-1 antibody
Overlay histogram showing NIH/3T3 (Mouse embryo fibroblast cell line) cells stained with ab695 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab695, 2 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab695 は 15 報の論文で使用されています。
- Lim SC et al. Extracellular acidity-induced expression of Kallikrein-related peptidases 7 and 8 is involved in increased invasiveness of gastric cancer cells. Oncol Rep 43:1705-1713 (2020). PubMed: 32323843
- Chen WY et al. Androgen deprivation-induced ZBTB46-PTGS1 signaling promotes neuroendocrine differentiation of prostate cancer. Cancer Lett 440-441:35-46 (2019). PubMed: 30312731
- Xiao M et al. Impact of the Chinese herbal medicines on dual antiplatelet therapy with clopidogrel and aspirin: Pharmacokinetics and pharmacodynamics outcomes and related mechanisms in rats. J Ethnopharmacol 235:100-110 (2019). PubMed: 30710735
- Cianciaruso C et al. Molecular Profiling and Functional Analysis of Macrophage-Derived Tumor Extracellular Vesicles. Cell Rep 27:3062-3080.e11 (2019). PubMed: 31167148
- Ding L et al. Downregulation of cyclooxygenase-1 stimulates mitochondrial apoptosis through the NF-?B signaling pathway in colorectal cancer cells. Oncol Rep 41:559-569 (2019). PubMed: 30320345