Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (carotid body)
Permeabilization
Yes - 0.5% TritonX in PBS
Specification
carotid body
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
4% PFA
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in 0.1% Triton X PBS
Dilution
1/1000
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Cy™3 AffiniPure Donkey Anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Additional data
Additional Notes
The carotid body glomus cells show positive staining in the cytoplasm region.
We have received code for the abtrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 06 2016