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  1. Link

    cox-iv-antibody-mitochondrial-loading-control-ab16056.pdf

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Tags & Cell Markers Subcellular Markers Organelles Mitochondria
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Anti-COX IV 抗体 - Mitochondrial Loading Control (ab16056)

  • Datasheet
  • SDS
Reviews (32)Q&A (19)References (216)

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Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
  • Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
  • Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
  • Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
  • Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)

Key features and details

  • Rabbit polyclonal to COX IV - Mitochondrial Loading Control
  • Suitable for: IHC-Fr, WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human, Xenopus laevis, Potato, African green monkey, Chinese hamster
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-COX IV antibody - Mitochondrial Loading Control
    COX IV 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to COX IV - Mitochondrial Loading Control
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Human, Xenopus laevis, Potato, African green monkey, Chinese hamster
    交差が予測される動物種: Chimpanzee, Monkey
  • 免疫原

    Synthetic peptide corresponding to Human COX IV aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab16381)

  • 特記事項

    This antibody makes an effective loading control for mitochondria. COX IV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COX IV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Mouse monoclonal to COX IV antibody [20E8] (ab14744).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 一次抗体 備考

    This antibody makes an effective loading control for mitochondria. COX IV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COX IV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Mouse monoclonal to COX IV antibody [20E8] (ab14744).
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Isotype/Loading Controls
    • Loading Controls
    • COX IV - Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex IV
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human COX IV protein (ab112273)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab16056の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr (2)
Use at an assay dependent concentration.
WB (15)
Use a concentration of 0.5 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).
ICC/IF (5)
Use a concentration of 1 µg/ml.
IHC-P (5)
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
IHC-Fr
Use at an assay dependent concentration.
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • 組織特異性

    Ubiquitous.
  • 配列類似性

    Belongs to the cytochrome c oxidase IV family.
  • 細胞内局在

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession P13073 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 454391 Chimpanzee
    • Entrez Gene: 1327 Human
    • Entrez Gene: 12857 Mouse
    • Entrez Gene: 29445 Rat
    • Omim: 123864 Human
    • SwissProt: O46577 Chimpanzee
    • SwissProt: P13073 Human
    • SwissProt: P19783 Mouse
    • SwissProt: P10888 Rat
    • Unigene: 433419 Human
    • Unigene: 386758 Mouse
    • Unigene: 2528 Rat
    see all
  • 別名

    • AL024441 antibody
    • COX 4 antibody
    • COX IV 1 antibody
    • COX IV antibody
    • COX IV-1 antibody
    • Cox4 antibody
    • COX41_HUMAN antibody
    • Cox4a antibody
    • COX4B antibody
    • COX4I1 antibody
    • COX4I2 antibody
    • COX4L2 antibody
    • COXIV antibody
    • Cytochrome c oxidase polypeptide IV antibody
    • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
    • Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody
    • Cytochrome C Oxidase subunit IV antibody
    • Cytochrome c oxidase subunit IV isoform 1 antibody
    • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
    • Cytochrome c oxydase subunit 4 antibody
    • dJ857M17.2 antibody
    • MGC105470 antibody
    • MGC72016 antibody
    see all

画像

  • Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
    Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
    All lanes : Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate
    Lane 2 : Rat skeletal muscle tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    Blocking buffer: 2% BSA

    Gel type: MES

    Exposure Time: 1 minute

  • Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
    Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
    Lane 1 : Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1 µg/ml (Human skeletal muscle tissue lysate)
    Lanes 2-3 : Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1 µg/ml

    Lane 2 : Human heart tissue lysate
    Lane 3 : Mouse skeletal muscle tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    Blocking buffer: 2% BSA

    Gel type: MES

    Exposure Time: 1 minute

  • Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
    Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
    All lanes : Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : Human heart tissue lysate - total protein (ab29431)
    Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)This image is courtesy of an anonymous Abreview

    ab16056 staining COX IV in Mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/600 ) for 12 hours at 4°C. A Cy5® donkey anti-rabbit secondary antibody was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
    Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)

    ab16056 stained in Hela cells. Cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab16056 at 1µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature

  • Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
    Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
    All lanes : Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 0.38 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Human skeletal muscle cell lysate
    Lane 3 : HeLa whole cell lysate with Human COX IV peptide (ab16381) at 1 µg/ml
    Lane 4 : Human skeletal muscle cell lysate with Human COX IV peptide (ab16381) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution

    Predicted band size: 17 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
    IHC image of COXIV staining in human liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16056, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)This image is courtesy of an anonymous Abreview.

    ab16056 staining COX IV in breast tumour tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% buffered formalin and blocked with 5% serum for 60 minutes at 21°C; antigen retrieval was by heat mediation in a 10mM sodium citrate buffer pH6. Samples were incubated with primary antibody (1/300 in blocking buffer) for 12 hours at 4°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (216)

ab16056 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab16056 は 216 報の論文で使用されています。

  • Fujita Y  et al. Functional Expression of Choline Transporters in Human Neural Stem Cells and Its Link to Cell Proliferation, Cell Viability, and Neurite Outgrowth. Cells 10:N/A (2021). PubMed: 33672580
  • Lagoutte-Renosi J  et al. Influence of Antiplatelet Agents on the Lipid Composition of Platelet Plasma Membrane: A Lipidomics Approach with Ticagrelor and Its Active Metabolite. Int J Mol Sci 22:N/A (2021). PubMed: 33572690
  • Acoba MG  et al. The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism. Cell Rep 34:108869 (2021). PubMed: 33730581
  • Wang J  et al. Regulatory role of DEPTOR-mediated cellular autophagy and mitochondrial reactive oxygen species in angiogenesis in multiple myeloma. Int J Mol Med 47:643-658 (2021). PubMed: 33416146
  • Sighel D  et al. Inhibition of mitochondrial translation suppresses glioblastoma stem cell growth. Cell Rep 35:109024 (2021). PubMed: 33910005
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 51 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-COX IV antibody - Mitochondrial Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Mar 09 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-COX IV antibody - Mitochondrial Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Mar 09 2021

Immunocytochemistry abreview for Anti-COX IV antibody - Mitochondrial Loading Control

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Rat Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - 0.02% Triton-X-100
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 May 07 2019

Immunocytochemistry abreview for Anti-COX IV antibody - Mitochondrial Loading Control

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (HEK293)
Permeabilization
Yes - 0.02% Triton-X-100
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 May 07 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-COX IV antibody - Mitochondrial Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (heart)
Permeabilization
Yes - 0.2% triton X
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Sep 07 2018

Western blot abreview for Anti-COX IV antibody - Mitochondrial Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (heart)
Gel Running Conditions
Non-reduced Denaturing (15%)
Loading amount
20 µg
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

投稿 Sep 07 2018

Western blot abreview for Anti-COX IV antibody - Mitochondrial Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (SH-SY5Y)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
SH-SY5Y
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

MR. Chun Chau SUNG

Verified customer

投稿 May 23 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-COX IV antibody - Mitochondrial Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (BS-C-1 cells)
Permeabilization
Yes - 0.2% Triton X
Specification
BS-C-1 cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Mr. J. Y. Seo

Verified customer

投稿 Apr 11 2018

Question

Inquiry: I have the answer on one of the question for the Ab16056 namely "Are these antibodies tested with Drosophila species?" . I've used this antibody for immunofluorescence staining in S2 cells yesterday and I have got pictures showing this Ab works very well in Drosophila. Best regards,

Read More

Abcam community

Verified customer

Asked on May 08 2012

Answer

Thank you very much for your feedback.

We would like to encourage you to submit an Abreview about your experiments with ab16056 in drosophila. This information is very valuable for our customers when choosing an antibody.

You would earn Abpoints that can be redeemed asdiscount on a future purchase or Amazon gift vouchers.

We are looking forward to your Abreview and wish you continuing luck with your research

Read More

Abcam Scientific Support

Answered on May 08 2012

Question

Hi, this antibody panel, ab110413, recognizes complex I-V quite sufficiently. I was wondering if there is any difference between the MTCO1 (COX IV subunit I) at 40kDa and another COX IV loading control (ab16056) at 17kDa? Since COX IV is typically used for loading control in the mitochondrial western blot. I use that Ab for other mitochondrial protein analysis. But I am not sure if I can use COX IV in the case of evaluating MTCO1, since they are in some way the same COX IV, right? And why the molecular weight for the proteins recognized by both Abs are different? Thanks! Hailong

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Abcam community

Verified customer

Asked on Jul 16 2018

Answer

The antibodies detect different subunits of cytochrome c oxidase, which is also known as Complex IV, and which is abbreviated as CIV on the ab110413 datasheet (Total OXPHOS Rodent WB Antibody Cocktail).

The CIV (Complex IV) antibody in the panel ab110413 detects Compex IV subunit 1, also called COXI and MT-CO1, at ˜40kDa. This antibody is available separately as ab14705. The COX IV mitochondria loading control antibody ab16056 detects Complex IV subunit 4, also known as COXIV, at about 15kDa. Here are links to the UniProt records for the human proteins.

MT-CO1 https://www.uniprot.org/uniprot/P00395
COXIV https://www.uniprot.org/uniprot/P13073

Read More

Abcam Scientific Support

Answered on Jul 16 2018

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