Application
Western blot
Sample
Human Cell lysate - whole cell (Hematological cancer cell line lysates)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
20 µg
Specification
Hematological cancer cell line lysates
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/1000
Detection
Detection method
ECL
Exposure
5 second(s)
Bands
Specific: ~15 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 14 2020