Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (egg extract)
Loading amount
15 µg
Specification
egg extract
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST / 5% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
ECL
Exposure
1 hour(s), 30 minute(s) and 0 second(s)
Bands
Specific: 15 (weak) kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Anne-Lore Schlaitz
Verified customer
投稿 Aug 21 2008