Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
15 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% Milk in TBST
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 17 kDa Non-specific: none kDa
Additional data
Additional Notes
M: Marker
1: cytosol
2: mitochondrial fraction
3: ER fraction
4: nucleus
1: cytosol
2: mitochondrial fraction
3: ER fraction
4: nucleus
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 17 2010