Anti-COX IV 抗体 [20E8C12] (ab14744)

Vielen Dank für Ihren Anruf.

Ich freue mich Ihnen bestätigen zu können, dass die Klone dieselben sind und früher bei MitoScience nur abgekürzt wurden.

Ich kann Ihnen auch bestätigen, dass eine Lagerung bei -20C korrekt ist solange die Antikörper lyophilisiert sind.

Bitte lagern sie die Antikörper bei +4C nach Rekonstitution.

We would recommend the use of one of the following products for a loading control with zebrafish samples: AB15224 anti alpha Tubulin (https://www.abcam.com/alpha-tubulin-antibody-ab15246.html). This product has been featured in the following publication for use in WB on zebrafish (PubMed ID: 22581286). AB6046 anti beta Tubulin. This product has an excellent Abreview provided by a customer using in WB on zebrafish. This review is located at the following web address: https://www.abcam.com/beta-Tubulin-antibody-Loading-Control-ab6046-reviews.html?intabreviewid=15893 AB14744 anti- COX IV antibody (https://www.abcam.com/COX-IV-antibody-20E8C12-Mitochondrial-Loading-Control-ab14744-references.html). Another guaranteed product for use as loading control this product has been used in publication (PubMed ID: 18765295).

Thank you very much for your reply.

The common nuclear outer membrane proteins are nesprins, NAV3, and OPRS1. The nesprins and OPRS1 can also be found in the inner membrane or other organelles, but the NAV3 seems to be more specific to the outer membrane. Unfortunately we do not have any monoclonal antibodies to this target, just polyclonals such as ab101792-

https://www.abcam.com/nav3-antibody-n-terminal-ab101792.html

We do have monoclonal antibodies to nesprins, such as ab125931-

https://www.abcam.com/nesprin-1-antibody-mannes1a7a12-ab125931.html

Would either of these work for you?

I am sorry that we don't have more options at this time, but please let me know if there is anything else that I can help find for you, and I'll be happy to help.

Thank you very much for your enquiry.

I am sorry to hear that the nesprin and TOMM22 antibodies are not working as they should be. Could you tell me the catalog numbers of each of these antibodies, and also a description of the protocol that you're using? We do fully guarantee all of our products to work as stated on the datasheets, so I'd be happy to send replacements if applicable. Could you also send your original order or PO number?

If you'd like to continue probing for nesprin and TOMM22, we do have a couple of antibodies that work in Western blot with human and mouse samples-

https://www.abcam.com/TOMM22-antibody-ab57523.html

https://www.abcam.com/Nesprin3-antibody-ab74261.html

We also have several other antibodies that are targeted against mitochondrial or nuclear membrane-bound proteins that work in Western blot with mouse and human samples:

Mitochondrial

Prohibition antibody: https://www.abcam.com/Prohibitin-antibody-Mitochondrial-Marker-ab28172.html

MTCO1 antibody: https://www.abcam.com/MTCO1-antibody-1D6E1A8-Mitochondrial-Marker-ab14705.html

Nuclear

Lamin B1 antibodies: https://www.abcam.com/Lamin-B1-antibody-Nuclear-Envelope-Marker-ab16048.html

https://www.abcam.com/Lamin-B1-antibody-119D5-F1-Nuclear-Envelope-Marker-ab8982.html

Would one of these work for you?

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

Thank you very much for contacting us with your questions.

Will you be trying to isolate intact nuclei from mammalian cells? I'm not sure if there is an antibody that would be good for this, butthecell fractionation kit ab109719 that I mentioned beforemight be suitable. I'll need to check with the lab whether the nucleiu will still be intact using this kit, if that is your end goal.

To check the efficiency of a nuclear fractionation, I would recommend probing for either lamin B1 (66 kDa) or TATA binding protein (38 kDa). Both of these proteins will run higher than the histones but should be far enough away from the IgG heavy and light chains around 25 and 50 kDa. For mitochondria, I'd recommend a VDCA1/porin antibody (31 kDa), though this may be too close to the 25 kDa light chain band. You could also probe for COXIV(16 kDa) but this protein may be smaller than what you'd like. What species will your samples be from? I can find specific antibodies that are known to react with this species.

I'm not sure if you would be interested inthis, but we do have secondary antibodies that only bind to native primary antibodies so that the denatured chains won't be visible at 25 and 50 kDa following an IP. For anti-mouse IgG, we have ab131368-

https://www.abcam.com/mouse-IgG-VeriBlot-for-IP-secondary-antibody-HRP-ab131368.html

For anti-rabbit IgG, we have ab131366-

https://www.abcam.com/rabbit-IgG-VeriBlot-for-IP-secondary-antibody-HRP-ab131366.html

I look forward to hearing from you. Please let me know if you have any further questions or if there is anything else that we can do for you.

Unfortunately, we do not have a special "rat liver mitochondria loading control"but we have one antibody which may interest you:

ab16056 Anti-COX IV antibody - Mitochondrial Loading Control

https://www.abcam.com/COX-IV-antibody-Mitochondrial-Loading-Control-ab16056.html (or use the following: https://www.abcam.com/COX-IV-antibody-Mitochondrial-Loading-Control-ab16056.html).

This antibody has two very positive Abreviews in WB and rat, and has been demonstrated to work in liver, however, the latter wasin IHC and human. Nonetheless, I think this is the right one for you in this case.