Anti-COX IV 抗体 [20E8C12] (ab14744)
Key features and details
- Mouse monoclonal [20E8C12] to COX IV
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
-
製品名
Anti-COX IV antibody [20E8C12]
COX IV 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [20E8C12] to COX IV -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, WBmore details -
種交差性
交差種: Mouse, Rat, Cow, Human
交差が予測される動物種: Pig -
免疫原
Tissue, cells or virus corresponding to Cow COX IV.
-
ポジティブ・コントロール
- Human, bovine, murine and rat heart mitochondria.
-
特記事項
This antibody makes an effective loading control for mitochondria. COXIV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COXIV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Rabbit polyclonal to COX IV antibody (ab16056).
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
-
精製度
IgG fraction -
一次抗体 備考
This antibody makes an effective loading control for mitochondria. COXIV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COXIV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Rabbit polyclonal to COX IV antibody (ab16056). -
ポリ/モノ
モノクローナル -
クローン名
20E8C12 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14744の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
WB | (18) |
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa.
|
特記事項 |
---|
Flow Cyt
Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa. |
ターゲット情報
-
機能
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the cytochrome c oxidase IV family. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 1327 Human
- Entrez Gene: 12857 Mouse
- Entrez Gene: 29445 Rat
- Omim: 123864 Human
- SwissProt: P00423 Cow
- SwissProt: P13073 Human
- SwissProt: P19783 Mouse
- SwissProt: P10888 Rat
see all -
別名
- AL024441 antibody
- COX 4 antibody
- COX IV 1 antibody
see all
画像
-
All lanes : Anti-COX IV antibody [20E8C12] (ab14744) at 1/5000 dilution
Lane 1 : Cytoplasmic fraction mouse NIH/3T3 cell lysate
Lane 2 : Nuclear fraction mouse NIH/3T3 cell lysate
Secondary
All lanes : HRP conjugated goat anti-mouse antibody
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 16 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
This image is courtesy of an Abreview submitted by Camilla Skjerpen on 4 July 2005. -
Overlay histogram showing HeLa cells stained with ab14744 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14744, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
-
All lanes : Anti-COX IV antibody [20E8C12] (ab14744) at 1 µg/ml
Lane 1 : Isolated mitochondria from human heart at 5 µg
Lane 2 : Isolated mitochondria from bovine heart at 1 µg
Lane 3 : Isolated mitochondria from rat heart at 10 µg
Lane 4 : Isolated mitochondria from murine heart at 10 µg
Observed band size: 16 kDa why is the actual band size different from the predicted?
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (269)
ab14744 は 269 報の論文で使用されています。
- Aleo SJ et al. Drug repositioning as a therapeutic strategy for neurodegenerations associated with OPA1 mutations. Hum Mol Genet 29:3631-3645 (2021). PubMed: 33231680
- Shannon CE et al. Insulin resistance is mechanistically linked to hepatic mitochondrial remodeling in non-alcoholic fatty liver disease. Mol Metab 45:101154 (2021). PubMed: 33359401
- Esparza-Moltó PB et al. Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition. PLoS Biol 19:e3001252 (2021). PubMed: 33983919
- Pan Y et al. Mitochondrial proteomics alterations in rat hearts following ischemia/reperfusion and diazoxide post-conditioning. Mol Med Rep 23:N/A (2021). PubMed: 33355377
- Yang K et al. Dopamine receptor D1 agonist inhibits glioblastoma via calpain-mediated ER stress and mitochondrial dysfunction. Oncol Rep 45:N/A (2021). PubMed: 33760205