Cortisol ELISA Kit (ab108674)
Key features and details
- Sensitivity: 2.95 ng/ml
- Range: 10 ng/ml - 500 ng/ml
- Sample type: Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
製品の概要
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製品名
Cortisol ELISA Kit
Cortisol キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Urine 60 <=6.5% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Urine 30 <=7.2% -
サンプルの種類
Urine -
アッセイタイプ
Competitive -
検出感度
2.95 ng/ml -
検出範囲
10 ng/ml - 500 ng/ml -
添加回収試験
107.48 %
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ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in urine.
A 96-well plate has been precoated with anti-Cortisol. Samples and the Cortisol-HRP conjugate are added to the wells, where Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Wash Solution 1 x 50ml Anti-Cortisol IgG Coated Microplate (12 x 8 wells) 1 unit Cortisol Standard 0 - 0 ng/mL 1 x 4ml Cortisol Standard 1 - 1 ng/mL 1 x 1ml Cortisol Standard 2 - 5 ng/mL 1 x 1ml Cortisol Standard 3 - 30 ng/mL 1 x 1ml Cortisol Standard 4 – 200 ng/mL 1 x 1ml Cortisol-HRP Conjugate 1 x 33ml Cover foils 1 unit High control 1 x 1ml Low control 1 x 1ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
研究分野
画像
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Typical Standard Curve
Representative Standard Curve using ab108674
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab108674 は 1 報の論文で使用されています。
- Muhie S et al. Molecular alterations induced by Yersinia pestis, dengue virus and Staphylococcal enterotoxin B under severe stress. Brain Behav Immun 80:725-741 (2019). PubMed: 31100372