Anti-Cortisol Binding Globulin 抗体 [EPR4615] - BSA and Azide free (ab247930)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4615] to Cortisol Binding Globulin - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cortisol Binding Globulin antibody [EPR4615] - BSA and Azide free
Cortisol Binding Globulin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4615] to Cortisol Binding Globulin - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab247930 is the carrier-free version of ab110648.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4615 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab247930の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa).
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa). |
ターゲット情報
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機能
Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species. -
組織特異性
Plasma; synthesized in liver. Has also been identified in a number of glycocorticoid responsive cells. -
関連疾患
Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency) [MIM:611489]. CBG deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo/hypertension and muscle fatigue. -
配列類似性
Belongs to the serpin family. -
翻訳後修飾
N-glycosylated; binds 5 oligosaccharide chains.
Glycosylation in position Asn-260 is needed for steroid binding. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 866 Human
- Omim: 122500 Human
- SwissProt: P08185 Human
- Unigene: 532635 Human
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別名
- CBG antibody
- CBG_HUMAN antibody
- corticosteroid binding globulin antibody
see all
画像
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All lanes : Anti-Cortisol Binding Globulin antibody [EPR4615] (ab110648) at 1/1000 dilution
Lane 1 : Human fetal artery lysate
Lane 2 : Human plasma lysate
Lane 3 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?This data was developed using ab110648, the same antibody clone in a different buffer formulation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab247930 は論文での使用が確認できていません。