Anti-Cortisol 抗体 [XM210] (ab1949)

Submit a review Q&A (6)References (11)

Key features and details

  • Mouse monoclonal [XM210] to Cortisol
  • Suitable for: ELISA
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-Cortisol antibody [XM210]
    Cortisol 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [XM210] to Cortisol

  • 由来種

    Mouse

  • 特異性

    Cortisol, Ka = 1.7 x 109 M-1. XM-210 has some cross-reactivity with cortisone (~0.6%)

  • アプリケーション

    適用あり: ELISAmore details

  • 免疫原

    Chemical/ Small Molecule corresponding to Cortisol conjugated to Bovine Serum Albumin (BSA).

  • 特記事項

    Concentration varies from lot to lot and can be provided on request.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.50
    Preservative: 0.05% Sodium azide
    Constituents: 0.121% Tris, 0.87% Sodium chloride
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 特記事項（精製）

    Purified by ion exchange chromatography and tested by electrophoresis.

  • ポリ/モノ

    モノクローナル

  • クローン名

    XM210

  • ミエローマ

    Sp2/0

  • アイソタイプ

    IgG2a

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

  • 細胞内局在

    Secreted

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (11)

ab1949 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1949 は 11 報の論文で使用されています。

  • Shin J  et al. Smart Fatigue Phone: Real-time estimation of driver fatigue using smartphone-based cortisol detection. Biosens Bioelectron 136:106-111 (2019). PubMed: 31048212
  • Mes D  et al. Neurobiology of Wild and Hatchery-Reared Atlantic Salmon: How Nurture Drives Neuroplasticity. Front Behav Neurosci 12:210 (2018). PubMed: 30254575
  • Höglund E  et al. Dietary l-tryptophan leaves a lasting impression on the brain and the stress response. Br J Nutr 117:1351-1357 (2017). PubMed: 28625179
  • van den Bos R  et al. Further characterisation of differences between TL and AB zebrafish (Danio rerio): Gene expression, physiology and behaviour at day 5 of the larval stage. PLoS One 12:e0175420 (2017). RIA . PubMed: 28419104
  • Munje RD  et al. A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs). Sci Rep 7:1950 (2017). PubMed: 28512341
View all Publications for this product

レビューと Q&A

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1-6 of 6 Abreviews or Q&A

Question

Are these antibodies cross reactive with dog cortisol and testosterone?

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Answer

To our knowledge, ab1949 nor ab6066 have not yet been tested in detecting canine cortisol and testosterone. However, as cortisol and testosterone are steroids, antibodies raised against them should detect them regardless of the species that they are produced in as their structure and composition should be the same.

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Question

Inquiry: Hi I require an anti-cortisol antibody which I can use to coat ELISA plates for both a diurnal cortisol assay and subsequently a cross reactant assay. The reaction will be detected by either OPD or TMB. Are any of your anti-cortisol antibodies suitable and which is the best

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Answer

Thank you for contacting us and your interest in our products.

We have two antibodies directed against cortisol which have been used in ELISA previously. These are the mouse monolonals ab1949 and ab116600. However these have not been used in a sandwich ELISA format or "diurnal cortisol assay and subsequently a cross reactant assay" as you have described and we would therefore not be able to guarantee that they would be suitable for this experiment. If you could describe in a bit more detail how you intend to use the antibody I may be able to comment further on whether these antibodies are likely to work in this setting.

Alternatively we have ELISA kits designed to detect cortisol in human serum or plasma (ab108665), urine (ab108674) or saliva (ab154996) which may be of interest to you. These are based on a competitive immunoenzymatic colorimetric method intended for the quantitative determination of Cortisol.

I hope this information has been helpful. If I can be of any further assistance, please do not hesitate to let me know.

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Question

Hi,

Thank you for your email.

Sorry our customer and I forgot to mentioned ELISA as the application.

Let me know if you need any further info.

Kind regards,

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Answer

Thank you for confirming that information.

The antibodies you have suggested for DHEA (ab54202, ab35062 and ab123987) should be fine for what the customer has requested.

The same is true of the anti-DHEA-S (ab31668). The anti-Cortisol antibodies ab1949 and ab116600 can also be suggested. The anti-Androstendione you have suggested should also be suitable (ab34944).

We do also have an antibody raised to target the hydroxy-Pregnenolone (ab34916). I am not sure if this is what the customer is after but it may be worth them having a look.

The secondary antibody they need would depend on which antibodies they choose to buy in the end. We ahve a very good search engine which you can use to find suitable antibodies:

https://www.abcam.com/index.html?pageconfig=productmap&cl=918

All you need to do is put in the isotype of the primary antibody and the species they are raised in (available on the datasheet of the primary antibody), specify that it is to be used in ELISA and that it is an HRP conjugate you want.

If you want further help with this, please let me know which antibodies the customer decides to try.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to contact me again.

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Question

Our customer is looking forcortisol antibody and I have attached reference.

I searched one ofcortisol antibody from abcam website.
https://www.abcam.com/Cortisol-antibody-XM210-ab1949.html

She is going to follow this test so I also searched Cortisol 3(CMO).

Please let me know if ab1949 is okay for this experiment.
Best regards

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Answer

Thank you for contacting us.

To be honest we do not have an antibody that we have tested with this kind of novel assay, and therefore do not know if it would work nor can we guarantee it.

Although ab1949 may work, I may recommend ab1952: It is also a mouse monoclonal, is guaranteed to work in competitive binding assays (which might be more related to the intended experimental approach) and crucially detects also free cortisol. Furthermore, there is no cross-reactivity with cortisone, if this is of importance to the customer. (ab1951 is similar, but it cross-reacts with cortisone).

https://www.abcam.com/Cortisol-antibody-CORT-2-ab1952.html (or use the following: https://www.abcam.com/Cortisol-antibody-CORT-2-ab1952.html).

https://www.abcam.com/Cortisol-antibody-CORT-1-ab1951.html (or use the following: https://www.abcam.com/Cortisol-antibody-CORT-1-ab1951.html).

If the customer considers testing this antibody in this application though, we would be very grateful to receive some feedback (probably once the results are published).

Also, we unfortunately do not have Cortisol-3-CMO-NHS ester or something similar available in our catalogue at the moment.

I am sorry that I cannot be of more assistance on this occasion, but I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Email: yvonne.couch@pharm.ox.ac.uk I would like to run a cortisol/hydrocortisone ELISA on mouse serum, does this product react with mouse serum?

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Answer

Thanks for your inquiry. Yes ab1949 should work in mouse serum. However, there will likely be an issue with non-specific binding of your secondary antibody, which will be an anti-mouse IgG, with the IgG in the mouse serum. Of course, this is assuming you are not doing a direct ELISA, which is probably the best way to test your sample to eliminate the secondary antibody issues. I hope this answers your question. Please feel free to contact me if you require further assistance.

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Question

I think that I asked the wrong question. At what position is BSA attached to the immunogen? Thank you

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Answer

Thank you for your enquiry. The immunogen is Cortisol-3(CMO)-BSA. Please do not hesitate to contact us if you need anything further.

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