製品の概要

  • 製品名

    Anti-Cortisol antibody [CORT-2]
    Cortisol 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [CORT-2] to Cortisol
  • 由来種

    Mouse
  • 特異性

    Cortisol-BSA conjugate and free cortisol. There is no cross-reactivity with BSA.
  • アプリケーション

    適用あり: competitive binding assaysmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Chemical/ Small Molecule corresponding to Cortisol conjugated to bovine serum albumin.

  • 特記事項

    Concentration varies from lot to lot and can be provided on request.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度

    Ascites
  • 特記事項(精製)

    Purified from ascites.
  • ポリ/モノ

    モノクローナル
  • クローン名

    CORT-2
  • ミエローマ

    Sp2/0
  • アイソタイプ

    IgG3
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
competitive binding assays Use at an assay dependent dilution.

ターゲット情報

  • 関連性

    Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.
  • 細胞内局在

    Secreted

画像

  • Cross-reactivity, % 50% displacement of cortisol-3-125-Iodine.

参考文献

This product has been referenced in:

  • Chen SR  et al. Disruption of genital ridge development causes aberrant primordial germ cell proliferation but does not affect their directional migration. BMC Biol 11:22 (2013). IHC . Read more (PubMed: 23497137) »
  • Qian X  et al. Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in adult rat testes. Endocrinology 154:1907-20 (2013). Read more (PubMed: 23546604) »
See all 3 Publications for this product

レビューと Q&A

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

To be honest we do not have an antibody that we have tested with this kind of novel assay, and therefore do not know if it would work nor can we guarantee it.

Although ab1949 may work, I may recommend ab1952: It is also a mouse monoclonal, is guaranteed to work in competitive binding assays (which might be more related to the intended experimental approach) and crucially detects also free cortisol. Furthermore, there is no cross-reactivity with cortisone, if this is of importance to the customer. (ab1951 is similar, but it cross-reacts with cortisone).

https://www.abcam.com/index.html?datasheet=1952 (or use the following: https://www.abcam.com/index.html?datasheet=1952).

https://www.abcam.com/index.html?datasheet=1951 (or use the following: https://www.abcam.com/index.html?datasheet=1951).

If the customer considers testing this antibody in this application though, we would be very grateful to receive some feedback (probably once the results are published).

Also, we unfortunately do not have Cortisol-3-CMO-NHS ester or something similar available in our catalogue at the moment.

I am sorry that I cannot be of more assistance on this occasion, but I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Cross-reaction of cortisol antibodies with cortisone is the following: ab1949 - 0.6 %, ab1951 - 35 % and ab1952 - 0 %.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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