Anti-COMT 抗体 [EPR6490] - BSA and Azide free (ab248128)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6490] to COMT - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-COMT antibody [EPR6490] - BSA and Azide free
COMT 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6490] to COMT - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248128 is the carrier-free version of ab126618.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6490 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248128の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa). |
ターゲット情報
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機能
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. -
組織特異性
Brain, liver, placenta, lymphocytes and erythrocytes. -
配列類似性
Belongs to the mammalian catechol-O-methyltransferase family. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Cytoplasm and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1312 Human
- Entrez Gene: 12846 Mouse
- Entrez Gene: 24267 Rat
- Omim: 116790 Human
- SwissProt: P21964 Human
- SwissProt: O88587 Mouse
- SwissProt: P22734 Rat
- Unigene: 370408 Human
see all -
別名
- Catechol O methyltransferase antibody
- Catechol O-methyltransferase antibody
- COMT antibody
see all
画像
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All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution (Purified)
Lane 1 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : Rat spleen lysate
Lane 4 : Rat kidney lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 30 kDa -
All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : COMT knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 24-28 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab126618).
Lanes 1-2: Merged signal (red and green). Green - ab126618 observed at 24-28 kDa. Red - loading control ab8245 observed at 37 kDa.
ab126618 Anti-COMT antibody [EPR6490] was shown to specifically react with COMT in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266537 (knockout cell lysate ab257396) was used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. ab126618 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248128 は論文での使用が確認できていません。