製品の概要

  • 製品名
    Complex II WB Antibody Cocktail
    Complex II キット 製品一覧
  • アッセイタイプ
    Quantitative
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 製品の概要

    Contains a total of 305 µg of antibodies and is a pre-mixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V alpha as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in cell lines relative to the control protein (Complex V alpha).


    Cocktail Antibodies:


    Mouse C-V-alpha [15H4C4] monoclonal:


    Amount: 50 µg


    Working concentration: 1 µg/ml


    Mouse C-II-30 (FeS) [21A11AE7] monoclonal:


    Amount: 250 µg


    Working concentration: 5 µg/ml


    Mouse C-II-70 (FL) [2E3GC12FB2AE2] monoclonal:


    Amount: 5 µg


    Working concentration: 0.1 µg/ml


     

  • 特記事項

    Related products

    Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

  • アプリケーション
    適用あり: WBmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 6 µg/ml. The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6 µg/mL for Western blotting.

画像

  • All lanes : Complex II WB Antibody Cocktail (ab110410) at 1/250 dilution

    Lane 1 : human heart mitochondria at 10 µg
    Lane 2 : Immunoprecipitated Complex II at 0.5 µg


    The Western blot cocktail ab110410 contains two Complex II subunits SDHA (70 kDa) and SDHB (30 kDa)and a general mitochondrial marker ATP synthase alpha subunit (F1α, 55 kDa). All three are present in the mitochondrial sample (1). The Complex II sample purified by Complex II immunoprecipitation beads (ab109799) shows only Complex II subunits (2).

参考文献

This product has been referenced in:
  • Ma S & Dong Z Melatonin Attenuates Cardiac Reperfusion Stress by Improving OPA1-Related Mitochondrial Fusion in a Yap-Hippo Pathway-Dependent Manner. J Cardiovasc Pharmacol 73:27-39 (2019). Read more (PubMed: 30418242) »
  • Hou Y  et al. Genetic ablation of TAZ induces HepG2 liver cancer cell apoptosis through activating the CaMKII/MIEF1 signaling pathway. Onco Targets Ther 12:1765-1779 (2019). Read more (PubMed: 30881030) »
See all 11 Publications for this product

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