Anti-Complement factor B 抗体 [EPR9288(B)] (ab133765)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9288(B)] to Complement factor B
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Complement factor B antibody [EPR9288(B)]
Complement factor B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9288(B)] to Complement factor B -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human serum and plasma
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
解離定数(KD 値)
KD = 1.43 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR9288(B) -
アイソタイプ
IgG
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133765の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 95, 60 kDa (predicted molecular weight: 86 kDa).
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 95, 60 kDa (predicted molecular weight: 86 kDa). |
ターゲット情報
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関連性
Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. -
細胞内局在
Plasma membrane and Secreted -
参照データベース
- Entrez Gene: 629 Human
- Omim: 138470 Human
- SwissProt: P00751 Human
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別名
- C3/C5 convertase antibody
- Complement factor B antibody
- Glycine-rich beta glycoprotein antibody
see all
画像
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All lanes : Anti-Complement factor B antibody [EPR9288(B)] (ab133765) at 1/1000 dilution
Lane 1 : Human serum
Lane 2 : Human plasma
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 86 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa (possible isoform)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab133765 は 2 報の論文で使用されています。
- He C et al. Low CFB expression is independently associated with poor overall and disease-free survival in patients with lung adenocarcinoma. Oncol Lett 21:478 (2021). PubMed: 33968194
- Hayashi N et al. Multiple biomarkers of sepsis identified by novel time-lapse proteomics of patient serum. PLoS One 14:e0222403 (2019). PubMed: 31568522