Anti-Collagen X 抗体 (ab58632)
Key features and details
- Rabbit polyclonal to Collagen X
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Collagen X antibody
Collagen X 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Collagen X -
由来種
Rabbit -
特異性
ab58632 recognizes type X collagen. Exhibits slight cross-reactivity with fibronectin and type II and type IX collagen. Does not cross-react with type I, type III, or type XI collagen. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) Type X collagen from rat chondrosarcoma cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58632の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/100 - 1/300.
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特記事項 |
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WB
1/100 - 1/300. |
ターゲット情報
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機能
Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage. -
関連疾患
Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity. -
配列類似性
Contains 1 C1q domain. -
翻訳後修飾
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 1300 Human
- Omim: 120110 Human
- SwissProt: Q03692 Human
- Unigene: 520339 Human
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別名
- COAA1_HUMAN antibody
- Col10a 1 antibody
- COL10A1 antibody
see all
画像
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Anti-Collagen X antibody (ab58632) at 1/500 dilution + HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 64,66 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 50 kDa, 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 seconds
The band observed at 64 kDa could potentially be a cleaved form of Collagen X due to the presence of a 18 amino acid signal peptide.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (227)
ab58632 は 227 報の論文で使用されています。
- Wu C et al. Mapk7 deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling. Genes Dis 11:964-977 (2024). WB ; Mouse . PubMed: 37692479
- Li Y et al. Enhancing cartilage repair with optimized supramolecular hydrogel-based scaffold and pulsed electromagnetic field. Bioact Mater 22:312-324 (2023). PubMed: 36263100
- Zhou W et al. High expression COL10A1 promotes breast cancer progression and predicts poor prognosis. Heliyon 8:e11083 (2022). PubMed: 36281404
- Gomez GA et al. Contrasting effects of Ksr2, an obesity gene, on trabecular bone volume and bone marrow adiposity. Elife 11:N/A (2022). PubMed: 36342465
- Ma Z et al. Mechanical Unloading of Engineered Human Meniscus Models Under Simulated Microgravity: A Transcriptomic Study. Sci Data 9:736 (2022). PubMed: 36450785