Key features and details
- Rabbit polyclonal to Collagen VI
- Suitable for: WB
- Reacts with: Cow, Human
- Isotype: IgG
製品名Anti-Collagen VI antibody
Collagen VI 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Collagen VI
特異性Negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
アプリケーション適用あり: WBmore details
種交差性交差種: Cow, Human
交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Dog, Pig, Mammals
Full length native protein (purified) corresponding to Human Collagen VI aa 1-1028. Collagen Type VI from human and bovine placenta.
Database link: P12109
- WB: HepG2 whole cell lysate.
At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファーPreservative: 0.01% Sodium azide
Constituents: 0.44% Sodium chloride, 4.8% Sodium borate, 0.15% EDTA
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Our Abpromise guarantee covers the use of ab6588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 108 kDa).
This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.
機能Collagen VI acts as a cell-binding protein.
関連疾患Defects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy.
配列類似性Belongs to the type VI collagen family.
Contains 3 VWFA domains.
翻訳後修飾Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
細胞内局在Secreted > extracellular space > extracellular matrix.
- Information by UniProt
- Alpha 1 (VI) chain (61 AA) antibody
- CO6A1_HUMAN antibody
- COL6A1 antibody
Anti-Collagen VI antibody (ab6588) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 108 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
ab6588 は 84 報の論文で使用されています。
- Jones JEC et al. The Adipocyte Acquires a Fibroblast-Like Transcriptional Signature in Response to a High Fat Diet. Sci Rep 10:2380 (2020). PubMed: 32047213
- Harish P et al. Inhibition of Myostatin Reduces Collagen Deposition in a Mouse Model of Oculopharyngeal Muscular Dystrophy (OPMD) With Established Disease. Front Physiol 11:184 (2020). PubMed: 32194441
- Semple C et al. Partial Weight-Bearing in Female Rats: Proof of Concept in a Martian-Gravity Analog. Front Physiol 11:302 (2020). PubMed: 32308630
- Bolduc V et al. Pathogenic variants in COL6A3 cause Ullrich-like congenital muscular dystrophy in young Labrador Retriever dogs. Neuromuscul Disord 30:360-367 (2020). PubMed: 32439203
- Mortreux M et al. Longitudinal time course of muscle impairments during partial weight-bearing in rats. NPJ Microgravity 5:20 (2019). PubMed: 31453318