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    collagen-vi-antibody-ab6588.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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Anti-Collagen VI 抗体 (ab6588)

  • Datasheet
  • SDS
Reviews (21)Q&A (28)References (91)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Collagen VI antibody (ab6588)

    Key features and details

    • Rabbit polyclonal to Collagen VI
    • Suitable for: WB
    • Reacts with: Cow, Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-Collagen VI antibody [EPR17072] (ab182744)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-Collagen VI antibody
      Collagen VI 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to Collagen VI
    • 由来種

      Rabbit
    • 特異性

      Negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
    • アプリケーション

      適用あり: WBmore details
    • 種交差性

      交差種: Cow, Human
      交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Dog, Pig, Mammals
    • 免疫原

      Full length native protein (purified) corresponding to Human Collagen VI aa 1-1028. Collagen Type VI from human and bovine placenta.
      Database link: P12109

    • ポジティブ・コントロール

      • WB: HepG2 whole cell lysate.
    • 特記事項

      At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • バッファー

      Preservative: 0.01% Sodium azide
      Constituents: 0.44% Sodium chloride, 4.8% Sodium borate, 0.15% EDTA
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • 特記事項(精製)

      Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Proteins
      • Collagen
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • Other

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Native Cow Collagen VI protein (ab7531)
      • Native Human Collagen VI protein (ab7538)

    アプリケーション

    Our Abpromise guarantee covers the use of ab6588 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 108 kDa).

    This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

    ターゲット情報

    • 機能

      Collagen VI acts as a cell-binding protein.
    • 関連疾患

      Defects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
      Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy.
    • 配列類似性

      Belongs to the type VI collagen family.
      Contains 3 VWFA domains.
    • 翻訳後修飾

      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    • 細胞内局在

      Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P12109 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 1291 Human
      • Entrez Gene: 1292 Human
      • Entrez Gene: 1293 Human
      • Entrez Gene: 12833 Mouse
      • Entrez Gene: 12834 Mouse
      • Entrez Gene: 12835 Mouse
      • Entrez Gene: 294337 Rat
      • Entrez Gene: 361821 Rat
      • Omim: 120220 Human
      • Omim: 120240 Human
      • Omim: 120250 Human
      • SwissProt: P12109 Human
      • SwissProt: P12110 Human
      • SwissProt: P12111 Human
      • SwissProt: Q02788 Mouse
      • SwissProt: Q04857 Mouse
      • Unigene: 474053 Human
      • Unigene: 2509 Mouse
      • Unigene: 232118 Rat
      see all
    • 別名

      • Alpha 1 (VI) chain (61 AA) antibody
      • CO6A1_HUMAN antibody
      • COL6A1 antibody
      • COL6A2 antibody
      • COL6A3 antibody
      • collagen 6 antibody
      • Collagen alpha 2(VI) chain antibody
      • Collagen alpha 3(VI) chain antibody
      • Collagen alpha-1(VI) chain antibody
      • collagen six antibody
      • Collagen type VI alpha 1 antibody
      • Collagen type VI alpha 2 antibody
      • Collagen type VI alpha 3 antibody
      • Collagen VI alpha 1 polypeptide antibody
      • Collagen VI alpha 2 polypeptide antibody
      • Collagen VI alpha 3 polypeptide antibody
      • CollagenVI antibody
      • Human mRNA for collagen VI alpha 2 C terminal globular domain antibody
      • OPLL antibody
      • PP3610 antibody
      see all

    画像

    • Western blot - Anti-Collagen VI antibody (ab6588)
      Western blot - Anti-Collagen VI antibody (ab6588)
      Anti-Collagen VI antibody (ab6588) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 108 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?

    プロトコール

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (91)

    ab6588 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6588 は 91 報の論文で使用されています。

    • Jones JEC  et al. The Adipocyte Acquires a Fibroblast-Like Transcriptional Signature in Response to a High Fat Diet. Sci Rep 10:2380 (2020). PubMed: 32047213
    • Harish P  et al. Inhibition of Myostatin Reduces Collagen Deposition in a Mouse Model of Oculopharyngeal Muscular Dystrophy (OPMD) With Established Disease. Front Physiol 11:184 (2020). PubMed: 32194441
    • Semple C  et al. Partial Weight-Bearing in Female Rats: Proof of Concept in a Martian-Gravity Analog. Front Physiol 11:302 (2020). PubMed: 32308630
    • Bolduc V  et al. Pathogenic variants in COL6A3 cause Ullrich-like congenital muscular dystrophy in young Labrador Retriever dogs. Neuromuscul Disord 30:360-367 (2020). PubMed: 32439203
    • Farini A  et al. PTX3 Predicts Myocardial Damage and Fibrosis in Duchenne Muscular Dystrophy. Front Physiol 11:403 (2020). PubMed: 32508664
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 49 Abreviews or Q&A

    Question

    Thanks for your kindly reply, after contact with this customer, she would like to have a credit note,

    could you please help her to create a credit note ?



    I deeply appreciate your great assistance!



    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Sep 06 2012

    Answer

    Thank you for contacting us.

    Your credit note ID is 22524.

    I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

    (1) Redeemed against the original invoice if this hasn't already been paid.
    (2) Held on the account for use against a future order.
    (3) A full refund can be offered where no other invoices are outstanding.

    Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

    To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

    The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

    Read More

    Abcam Scientific Support

    Answered on Sep 06 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Equus Cell (Tenocyte)
    Permeabilization
    No
    Specification
    Tenocyte
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Adrian Djalali Cuevas

    Verified customer

    投稿 Oct 31 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Dermal Fibroblast)
    Permeabilization
    No
    Specification
    Dermal Fibroblast
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Adrian Djalali Cuevas

    Verified customer

    投稿 Sep 26 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue (Cardiac tissue)
    Permeabilization
    No
    Specification
    Cardiac tissue
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 May 31 2017

    IHC - Wholemount abreview for Anti-Collagen VI antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (intestine organoids)
    Specification
    intestine organoids
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 29 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (adipocyte)
    Permeabilization
    Yes - 0.05% TX-100
    Specification
    adipocyte
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 24 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Artery)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
    Permeabilization
    No
    Specification
    Artery
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 07 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue sections (artery)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
    Permeabilization
    No
    Specification
    artery
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 01 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: CC1
    Specification
    colon
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 20 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Collagen VI antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (artery)
    Permeabilization
    No
    Specification
    artery
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 29 2016

    1-10 of 49 Abreviews or Q&A

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