Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Endothelial in Matrigel)
Specification
Endothelial in Matrigel
Fixative
Formaldehyde
Permeabilization
Yes - Acetone
Other product details
Concentration
0.5 µg/ml
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Anti Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Concentration
5 µg/ml
Additional data
Additional Notes
Human Endothelial Cells were embedded in 3D Matrigel gel (murine Collagen type 4) and stained against human collagen type 4 (AB6311), green channel, showing the localization of the col4 at the cell-ECM interface and no signal is detected in the gel showing the non-recognition of the antibody to murine col4. Additionally, Negative control of mouse kidney reveals no fluorescence.
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投稿 Sep 25 2012