Western blot abreview for Anti-Collagen IV antibody

Good
Abreviews
Application
Western blot
Sample
Human Tissue lysate - other (Skin)
Gel Running Conditions
Reduced Denaturing
Loading amount
6 µg
Specification
Skin
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% - BSA - PBS - Tween(0.1%)
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Peroxidase-IgG Fraction Monoclonal Mouse Anti-Rabb
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 270 kDa Non-specific: 150, 120 kDa

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投稿 Sep 06 2018

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