Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen IV antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Tumour plugs)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Trypsin 0.1% in 0.1% CaCl2
Permeabilization
No
Specification
Tumour plugs
Blocking step
BSA (1%) + 10% Goat Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/500

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: Goat anti-Rabbit Alexa 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

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投稿 Mar 16 2017

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