Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen IV antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH 6.0
Sample
Dog Tissue sections (liver)
Specification
liver
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 30 minute(s) · Temperature: 37°C · Diluent: PBS plus 1xcasein
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: ImmPress universal
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

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投稿 Nov 18 2013

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