Application
Immunohistochemistry (Frozen sections)
Blocking step
Blocking One as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Human Tissue sections (cultured skin (NHEK, NB1RGB))
Specification
cultured skin (NHEK, NB1RGB)
Permeabilization
Yes - 0.1% TritonX-100
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 10% Blocking One in PBS(-)
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 555 goat anti-rabbit IgG(H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Dilution
1/200
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 15 2013