Western blot abreview for Anti-Collagen IV antibody

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Abreviews
Application
Western blot
Sample
Syrian Hamster Cell lysate - whole cell (BHK)
Loading amount
100 µg
Specification
BHK
Gel Running Conditions
Reduced Denaturing (7.5)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS-Tween (0.5%)

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit IgG (HRP)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL+
Exposure
10 second(s)
Bands
Specific: 300 kDa
Positive control
BHK cell extract

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投稿 Oct 30 2012

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