Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen IV antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (3T3)
Specification
3T3
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3 min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Other product details

Dilution
1/250
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 488 Goat anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

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投稿 Sep 26 2012

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