Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (preovulatory follicle and whole ovary)
Specification
preovulatory follicle and whole ovary
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: From Vecta
Other product details
Dilution
1/600
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Vectastain kit from Vector
Host species: Mouse
Clonality: Monoclonal
Conjugation: Alkaline Phosphatase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Alkaline Phosphatase
Additional data
Additional Notes
Antigens were retrieved by boiling with an antigen unmasking solution (Vector Laboratories, Burlingame, CA, USA) for 20 min at 120ºC in an autoclave and then cooled down in water for 5 min.
Picture : Section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.
Picture : Section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.
Ms. Birgitta Weijdegard
Verified customer
投稿 Oct 10 2006