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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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Anti-Collagen III 抗体 [FH-7A] (ab6310)

  • Datasheet
  • SDS
Reviews (13)Q&A (24)References (132)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)
  • Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [FH-7A] (ab6310)

Key features and details

  • Mouse monoclonal [FH-7A] to Collagen III
  • Suitable for: IHC-Fr, IHC-P, WB, ELISA, Dot blot, Indirect ELISA, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG1

こちらの製品もご検討ください

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一次抗体
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Anti-Collagen IV antibody [EPR20966] (ab214417)
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Anti-Calponin 1 antibody [EP798Y] - BSA and Azide free (ab216651)

関連製品

製品の概要

  • 製品名

    Anti-Collagen III antibody [FH-7A]
    Collagen III 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [FH-7A] to Collagen III
  • 由来種

    Mouse
  • 特異性

    ab6310 specifically recognizes collagen type III from human and rat origin. It does not recognize collagen types I, II, IV, V, VI and X.

  • アプリケーション

    適用あり: IHC-Fr, IHC-P, WB, ELISA, Dot blot, Indirect ELISA, ICC/IFmore details
  • 種交差性

    交差種: Rat, Human
  • 免疫原

    Full length native protein (purified) (Human).

  • ポジティブ・コントロール

    • IHC-P: Rat skin sections. IHC-Fr: Rat skin sections.
  • 特記事項

    Type III collagen, [a1(III)]3 ,is an approx. 300 kDa molecule, found predominantly in skin, blood vessels, liver, placenta, tongue, and thymus. Collagen type III forms cofibrils with type I and/or V collagens in a number of tissues of mesenchymal origin, such as skin, tendon, ligaments, and bone. This collagen type is involved, directly or indirectly in several genetic diseases, including Ehlers-Danlos type IV disease.

     

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.097% Sodium azide
    Constituent: Whole serum
  • Concentration information loading...
  • 精製度

    Tissue culture supernatant
  • ポリ/モノ

    モノクローナル
  • クローン名

    FH-7A
  • アイソタイプ

    IgG1
  • 研究分野

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Stem Cells
    • Mesenchymal Stem Cells
    • Chondrogenesis
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • Native Human Collagen III protein (ab7535)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6310の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P (10)
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration.
ELISA (2)
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
Indirect ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 25136258
特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
Indirect ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 25136258

ターゲット情報

  • 機能

    Collagen type III occurs in most soft connective tissues along with type I collagen.
  • 関連疾患

    Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
    Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
    Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
  • 配列類似性

    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • 翻訳後修飾

    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • 細胞内局在

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P02461 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 1281 Human
    • Entrez Gene: 84032 Rat
    • Omim: 120180 Human
    • SwissProt: P02461 Human
    • SwissProt: P13941 Rat
    • Unigene: 443625 Human
    • Unigene: 3247 Rat
    • 別名

      • Alpha 1 type III collagen antibody
      • Alpha1 (III) collagen antibody
      • CO3A1_HUMAN antibody
      • COL 3A1 antibody
      • COL3A1 antibody
      • Collagen alpha 1(III) chain antibody
      • Collagen alpha-1(III) chain antibody
      • Collagen III alpha 1 chain precursor antibody
      • Collagen III alpha 1 polypeptide antibody
      • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
      • Collagen type III alpha 1 antibody
      • Collagen type III alpha 1 chain antibody
      • Collagen type III alpha antibody
      • Collagen, fetal antibody
      • EDS4A antibody
      • Ehlers Danlos syndrome type IV, autosomal dominant antibody
      • Fetal collagen antibody
      • Type III collagen antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)

      Staining of formalin-fixed, paraffin-embedded rat skin with 1:4,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.

      This image was generated using the ascites version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)This image is courtesy of an Abreview submitted by Birgitta Weijdegard

      ab6310 at 1/600 diltuion staining preovulatory follicle and whole ovary tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigens were retrieved by boiling with an antigen unmasking solution for 20 min at 120ºC in an autoclave and then cooled down in water for 5 minutes. The tissue sections were formaldehyde fixed and incubated with the antibody for 1 hour. An alkaline phosphatase conjugated antibody was used as the secondary. The image shows a section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.

      This image was generated using the ascites version of the product.

      See Abreview

    • Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [FH-7A] (ab6310)
      Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [FH-7A] (ab6310)

      Staining of frozen rat skin sections with 1:8,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.

      This image was generated using the ascites version of the product.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (132)

    ab6310 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6310 は 132 報の論文で使用されています。

    • Wang B  et al. circNRIP1 facilitates keloid progression via FXR1-mediated upregulation of miR-503-3p and miR-503-5p. Int J Mol Med 47:N/A (2021). PubMed: 33649815
    • Chen J  et al. Potential mechanisms of Guizhi decoction against hypertension based on network pharmacology and Dahl salt-sensitive rat model. Chin Med 16:34 (2021). PubMed: 33906674
    • Bi X  et al. Collagen Cross-Linking Is Associated With Cardiac Remodeling in Hypertrophic Obstructive Cardiomyopathy. J Am Heart Assoc 10:e017752 (2021). PubMed: 33356379
    • Zhang H  et al. Long non-coding RNA XIST promotes the proliferation of cardiac fibroblasts and the accumulation of extracellular matrix by sponging microRNA-155-5p. Exp Ther Med 21:477 (2021). PubMed: 33767772
    • Ahmad SU  et al. Evaluation of Wound-Healing and Antioxidant Effects of Marantodes pumilum (Blume) Kuntze in an Excision Wound Model. Molecules 26:N/A (2021). PubMed: 33466302
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    11-20 of 37 Abreviews or Q&A

    Question

    Thank you for your detailed answer about the anti-collagen III antibody ab6310.

    That's a pity indeed that the validity on pig was initially a mistake but these things happen. I am a regular customer who appreciate very much your policy about customer feedback and reviewing, and I'll submit an abreview about IHC-Fr in pig skin after performing the few more tests discussed.

    In return, to answer your questions:

    - From what I've read and optimized so far on frozen sections with different kind of antibodies, the antigen retrieval step is usually not needed as the fixation time is very short.

    I don't have any pronase, and I'm not sure it's worth for me buying some only for a test, with no insurance the antibody will work. I can try fixing in acetone instead or formalin see if it changes something.

    - I will follow your advice and block with only donkey serum or BSA but not both at the same time

    - The secondary antibody works well, I've written reviews about ab90395 (collagen I) and ab6328 (fibronectin EDA), where I used the same anti-mouse Cy2 secondary antibody on similar pig skin frozen sections with good results.

    - I have performed the procedure on a couple of separate samples, and I did it twice on independent batches before contacting the technical service, to ensure the problem was from the antibody and not from my experimental procedure.

    I’d like to try the reference ab23445, as I also would like to do some western blotting. If this second one doesn't work, will I still be eligible to try the other one, or for a refund?

    Thank you very much

    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Jul 24 2012

    Answer

    Thank you foryour understanding and cooperation. The details provided enable us to closely monitor the quality of our products.

    I hope the suggestions may proove useful with ab6310. It may be worth trying citrate antigen retreival with the PFA fixationif you have the reagents. It is unusual to use antigen retreival with IHC-Fr, however laboratory tests do indicate that this can sometimes improve the results.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issuedthe alternativefree of charge replacement of ab23445with the order number 1132685.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. The ab23445 is tested and covered by the guarantee for IHC-Fr, WB and pig samples. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Jul 24 2012

    Question

    Hello,

    I have purchased the anti-collagen III antibody ab6310 a few months ago (order #1076197).

    However, after several attemps to optimise the experimental conditions, I haven't managed to make it work properly in IHC-Fr on pig skin cryosections



    Here are the conditions I have used for IHC-Fr:

    Pig skin cryosections, 12um thick, embedded in OCT

    Fixation in either formaldehyde 4% in PBS, or neutral buffer formalin for 10min at RT

    Permeabilisation in PBS 0.1% triton for 10min at RT

    Blocking in PBS, 5% donkey serum, 1%BSA, 0.01% tween for 1h at RT

    Incubation of primary antibody ab6310, diluted 1/500 in PBS , 1% donkey serum, 0.1%BSA, 0.01% tween overnight at 4C

    Secondary antibody: Cy2 donkey anti mouse 1/100 for 1h at RT

    Mounting in Prolong mounting media

    (this protocol worked fine for the collagen I antibody ab90395 for which I submitted a review)

    Results: No positive staining was observed in the dermis, either in unwounded skin or in the wound bed (where there should be even more Collagen III).

    Another of your anti-col III antibodies ab7778 displays a strong positive staining in human dermis in IHC-P, that’s the kind of signal I was expected.

    Could you tell me the experimental conditions you have been using to validate it in IHC-Fr (I was surprised to read a line about some antigen retrieval with pronase, which is usually not necessary with frozen sections, but maybe that’s needed here. However nothing is precised on the first page, in the applications section. Would you have specific technical advices to help me make it work?

    Also, in your scientific support section about this antibody, I have just read that this antibody wasn’t actually experimentally validated on pig samples but only predicted to work, based on sequence similarity. I wish I had seen it before so I could have at least asked for the testing offer while purchasing it.

    Have you got any feedback of these customers who were working on pig tissue, did they manage to make it work?

    I also saw that you offered them to try other antibodies references that may work on pig, have you got more data on these other antibodies?



    I look forward to hearing from you

    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Jul 23 2012

    Answer

    Thank you for taking the time to contact us with this information. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I have investigated our records for ab6310 and have contacted our source for this antibody. Regrettably,I can confirm that this antibody has not been tested on pig. I am not sure why this information was originally on our datasheet. The datasheet has been updated with the correct information and I sincerely apologize for any confusion. The quality of our antibodies and datasheets is very important to us, as is any feedback and data we receive. It is regrettable that the information we had on this particular antibody was not of the standard both we and our customers expect from our products.

    In this case I would be pleased to provide you with an immediatecredit note, free alternative replacement or refund.

    Alternatively, as the immunogen for the antibody has high alignment to the pig protein, you may like to consider trying a few suggestions first that I hope may help improve the results.I would also appreciate if you can confirm some further details, to help with our quality monitoring:

    1. As requested, we have some information regarding the antigen retreival: ab6310 is only tested on unfixed frozen sections in-house, and the antigen retrieval method is 0.1% pronase for 10 minutes. However we also have feedback from other researchers using this antibody successfully in IHC on FFPE tissue, and heat-mediated antigen retrieval for 20 minutes in pH 6 citrate buffer should work as well.

    2. We recommend not to mix blocking agents in one experiment. Try using 2% serum or 2% BSA alone to block.

    3. Is the current vial of secondary antibody working well with other primary antibodies?

    4. Try some fresh samples.

    I hope this information is helpful. We do have the following collagen III antibodies that are tested in IHC-Fr and pig. You may wish to consider trying one of these as a replacement:

    ab24119 Rabbit polyclonal Anti-Collagen III antibody
    Tested in: ELISA, Dot, IHC-Fr
    Reacts with: Human, Pig
    https://www.abcam.com/Collagen-III-antibody-ab24119.html (or use the following: https://www.abcam.com/Collagen-III-antibody-ab24119.html).

    ab23445Mouse monoclonalCollagen III antibody [1E7-D7/Col3]
    Tested in: WB, ELISA, IHC-Fr
    Reacts with: Human, Rat, Dog, Kangaroo, Pig
    https://www.abcam.com/Collagen-III-antibody-1E7-D7Col3-ab23445.html (or use the following: https://www.abcam.com/Collagen-III-antibody-1E7-D7Col3-ab23445.html).


    Should the suggestions not improve the results, please do not hesitate to contact me again with details of how you would like to proceed.

    Read More

    Abcam Scientific Support

    Answered on Jul 23 2012

    Question

    ab7778 und ab6310 :Schwein und Schaf

    Read More

    Abcam community

    Verified customer

    Asked on Jun 28 2012

    Answer

    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

    Unserem Wissen nach wurde ab7778 bisher noch nicht inSchwein und Schafund ab6310 nicht in Schaf getestet.

    Falls Sie dies selbst testen möchten, kann ich Ihnen zurzeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert des Antikörpers abdecken, und gegen eine erneute Bestellung von uns verrechnet werden.

    Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

    1.) Bestätigen Sie mir bitte,welchen Antikörper Sietesten möchten.

    2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

    3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

    4.) Testen Sie den Antikörper in Schaf (und/ oder Schwein).

    5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
    Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
    https://www.abcam.com/abreviews

    6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einihn mit einer neuen Bestellung verrechnen lassen (halten Sie bei der Bestellung bitte den Rabattcode). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

    Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

    Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
    https://www.abcam.com/collaborationdiscount

    Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

    Read More

    Abcam Scientific Support

    Answered on Jun 28 2012

    Question

    no bands under reduced, denaturing conditions with human samples
    is FOC lot (same problem before)
    sample prep: RIPA buffer
    Ab: 1/100
    loaded up to 100 ug protein
    secondary: OK

    Read More

    Abcam community

    Verified customer

    Asked on Apr 24 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab83829.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Apr 24 2012

    Question

    We are still unable to get a signal in blots, even after following Abcam's advice.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 10 2012

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Apr 10 2012

    Question


    Vielen Dank für Ihre schnelle Antwort.
    Es wäre sehr schön, wenn Sie den Antikörper direkt zu uns ins Institut liefern lassen könnten, bitte fügen Sie nur unbedingt die Interne Anschrift zur Adresse.
    Ich wünsche Ihnen ebenfalls schöne Ostern und danke Ihnen für Ihre Unterstützung.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 10 2012

    Answer

    Vielen Dank für Ihre Nachricht.

    Wie besprochen, habe ich eine kostenlose Ersatzlieferung mit ab34710für Sie in Auftrag gegeben. Sie hat die Referenznummer x und sollte in den nächsten Tagen direkt bei Ihnen im Labor ankommen.

    Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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    Abcam Scientific Support

    Answered on Apr 10 2012

    Question

    Anbei der ausgefüllte Fragebogen, ich hoffe, das hilft Ihnen weiter.
    Wäre es vielleicht möglich, statt des ab6310 den ab34710 Antikörper zu bekommen?
    Mit bestem Dank.

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    Abcam community

    Verified customer

    Asked on Apr 05 2012

    Answer

    Vielen Dank fürIhreNachricht und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

    Es tut mir leid, dass Sie Probleme mit unserem Collagen III Antikörper hatten. Nachdem ich mir die Details angesehen habe, stimme ich mit Ihnen überein, dass wohl in der Tat das Vial von ab6310, das Sie erhalten hatten, defekt ist. Wir möchten uns dafür entschuldigen.

    Gerne kann ich Ihnen zum Ersatz den Collagen I Antikörper ab34710kostenlos zukommen lassen! Soll ich ihn an die gleiche Adresse wie zuvor schicken ? Oder möchten Sie ihn direkt in Ihr Institut geliefert bekommen ?

    Vielen Dank für Ihre Kooperation und Hilfsbereitschaft. Ich freue mich bald wieder von Ihnen zu hören, und wünsche schon mal Frohe Ostern!

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    Abcam Scientific Support

    Answered on Apr 05 2012

    Question

    Liebe Mitarbeiter von Abcam,
    wir haben kürzlich einen Antikörper von Ihnen erhalten, der jedoch in unseren Händen kein Signal im Western-Blot aufweist. Es handelt sich um den Collagen III Antikörper ab6310 (https://www.abcam.com/Collagen-III-antibody-FH-7A-ab6310.html). Wir haben den Anikörper auf Zell- und Organlysaten getestet, parallel andere Blots entwickelt, die Signal aufwiesen und gehen nach mehrfachem Testen nun davon aus, dass es sich um Problem mit dem entsprechenden Tube handelt.
    Gibt es eine positive Kontrolle, die wir vielleicht verwenden können oder können Sie uns einen Ersatz zukommen lassen?
    Besten Dank für Ihre Unterstützung.

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    Abcam community

    Verified customer

    Asked on Apr 03 2012

    Answer

    Vielen Dank für Ihre Anfrage.

    Es tut mir leid zu hören, dass Sie Probleme mit diesem Collagen IIIAntikörper haben.

    Wenn der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben (und das scheint offensichtlich der Fall zu sein), haben Sie natürlich Anspruch auf unsere Garantie und können einen kostenlosen Ersatz von uns bekommen!

    Um dem Problem auf den Grund zu gehen und auch um wichtige Informationen für unsere Qualitätskontrolle zu erhalten, benötigen wir ein paar genauereInformationen über Ihre Proben und Ihr Protokoll. Ichhabe daher unseren Fragebogen als Word-Dokument an diese E-Mail angehängt und würde mich freuen, wenn Sie ihn ausfüllen würden und ggf. eine Abbildung beifügen könnten (vielleicht im Vergleich zu den anderen Antikörpern, die funktioniert haben?).

    Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und er innerhalb der letzten sechs Monate gekauft wurde, werden wir Ihnen dann gerne einen Ersatz oder eine Gutschrift schicken. Der Ersatz könnte jeder primäre Antikörper Ihrer Wahl sein (also zum Beispiel ein neues Röhrchen ab6310), oder ich könnte Ihnen eine Positivkontrolle zukommen lassen (zum Beispiel rekombinantes Collagen III ab7535: https://www.abcam.com/Native-Human-Collagen-III-protein-ab7535.html (or use the following: https://www.abcam.com/Native-Human-Collagen-III-protein-ab7535.html).)

    Ich freue mich, bald wieder von Ihnen zu hören.

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    Abcam Scientific Support

    Answered on Apr 03 2012

    Question

    ab6310 says "pig"when you search for all collagen II antibodies but does not mention "pig" reactivity on actual datasheet

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    Abcam community

    Verified customer

    Asked on Mar 22 2012

    Answer

    Thank you for contacting us. We are working on correcting the error for ab6310, as pig has not yet been tested. The immunogen for this product was human collagen III which ihas 93% sequence homology with the pig protein. We do not have any other collagen III antibodies tested for pig. The only other opetion might be ab7778, which is "predicted" to react in many mammals and has human and cow as the immunogen. The cow sequence is actually identical to pig so this would potentially work slightly better.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Abcam Scientific Support

    Answered on Mar 22 2012

    Question

    I would like to let you know that the collagen III antibody (ab7778) works very well on my paraffin-embedded mouse prostate tissue section.

    Thanks for your help.

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    Abcam community

    Verified customer

    Asked on Mar 09 2012

    Answer

    Thanks for keeping me updated abouttheprogress. I'm so glad to hear that ab7778 gave positive results!

    Please let me know if there is anything else that you might need from me in the future. Have a wonderful weekend!

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    Abcam Scientific Support

    Answered on Mar 09 2012

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