Anti-Collagen III 抗体 [EPR17673] (ab184993)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17673] to Collagen III
- Suitable for: Flow Cyt (Intra), ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Collagen III antibody [EPR17673]
Collagen III 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17673] to Collagen III -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, IP, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human fetal liver, fetal kidney, skin, skeletal muscle and heart lysates; HeLa, MCF7, HaCaT and A431 whole cell lysates. Mouse skeletal muscle tissue lysates, rat skeletal muscle tissue lysates, mouse heart tissue lysate, C6, RAW264.7, PC-12 and NIH/3T3 whole cell lysates. C6, RAW264.7 and PC-12 whole cell fresh lysate. ICC/IF: HeLa, MCF7, PC-12 and RAW 264.7 cells Flow Cyt (intra): HeLa, PC-12 and RAW 264.7 cells IP: HeLa whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17673 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184993の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/50.
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ICC/IF |
1/100.
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IP |
1/30.
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WB |
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
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特記事項 |
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Flow Cyt (Intra)
1/50. |
ICC/IF
1/100. |
IP
1/30. |
WB
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa). |
ターゲット情報
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機能
Collagen type III occurs in most soft connective tissues along with type I collagen. -
関連疾患
Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells. -
配列類似性
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain. -
翻訳後修飾
Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 1281 Human
- Entrez Gene: 12825 Mouse
- Entrez Gene: 84032 Rat
- Omim: 120180 Human
- SwissProt: P02461 Human
- SwissProt: P08121 Mouse
- SwissProt: P13941 Rat
- Unigene: 443625 Human
see all -
別名
- Alpha 1 type III collagen antibody
- Alpha1 (III) collagen antibody
- CO3A1_HUMAN antibody
see all
画像
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling Collagen III with ab184993 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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All lanes : Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution
Lane 1 : Human fetal liver lysate at 20 µg
Lane 2 : Human fetal kidney lysate at 20 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 5 : HaCaT (human keratinocyte cell line) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 139 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Exposure time : Lane 1: 3 minutes; Lane 2: 15 seconds; Lane 3: 3 seconds; Lanes 4-5: 30 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 26017148).
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Collagen III was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input).
Lane 2: ab184993 IP in HeLa whole cell lysate .
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184993 in HeLa whole cell lysate .
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5.5 seconds.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Collagen III with ab184993 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma small irregularly shaped cells) cell line labeling Collagen III with ab184993 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (ab150081) at 1/1000 dilution (green).Confocal image showing cytoplasmic staining in PC-12 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (ab150081) at 1/1000 dilution.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cell line labeling Collagen III with ab184993 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (ab150081) at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (ab150081) at 1/1000 dilution.
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Collagen III was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: RAW 264.7 whole cell lysate 10 μg (Input).
Lane 2: ab184993 IP in RAW 264.7 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184993 in RAW 264.7 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3.25 seconds.
Lysate was freshly made and used immediately to minimize protein degradation.
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All lanes : Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution
Lane 1 : Human skin lysate
Lane 2 : Human skeletal muscle lysate
Lane 3 : Human heart lysate
Lane 4 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 2-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 139 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1-3: 30 seconds; Lane 4: 5 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 26017148).
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All lanes : Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 5 : C6 (rat glial tumor glial cell), whole cell fresh lysate
Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell fresh lysate
Lane 7 : PC-12 (rat adrenal gland pheochromocytoma), whole cell fresh lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 139 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1-4: 92 seconds; Lanes 5-6: 48 seconds; Lane 7: 37 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
In lane 1-4, the lysates were stored at -80? prior to Western Blotting.
In lane 5-7, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
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All lanes : Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution
Lane 1 : Mouse skeletal muscle tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 139 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesExposure time : 3 minutes
Blocking/Dilution buffer: 5% NFDM/TBST.
-
Collagen III was immunoprecipitated from 0.35 mg of PC-12 (rat adrenal gland pheochromocytoma cell), whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: PC-12 whole cell lysate 10 μg (Input).
Lane 2: ab184993 IP in PC-12 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184993 in PC-12 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3.25 seconds.
Lysate was freshly made and used immediately to minimize protein degradation.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Collagen IIIwith ab184993 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cell line labeling Collagen III with ab184993 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150081) at 1/2000 dilution was used as the secondary antibody.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized PC-12 (Rat adrenal gland pheochromocytoma) cell line labeling Collagen III with ab184993 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150081) at 1/2000 dilution was used as the secondary antibody.
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Collagen III was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input).
Lane 2: ab184993 IP in HeLa whole cell lysate .
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184993 in HeLa whole cell lysate .
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (47)
ab184993 は 47 報の論文で使用されています。
- Liang L et al. cGAS exacerbates Schistosoma japonicum infection in a STING-type I IFN-dependent and independent manner. PLoS Pathog 18:e1010233 (2022). PubMed: 35108342
- Zhuang C et al. Sacubitril/Valsartan Improves Sexual Function and Fibrosis of the Clitoral and Vaginal Tissues in Female Spontaneously Hypertensive Rats. J Cardiovasc Pharmacol 79:858-872 (2022). PubMed: 35266909
- Liu B et al. Proximal tubular RAGE mediated the renal fibrosis in UUO model mice via upregulation of autophagy. Cell Death Dis 13:399 (2022). PubMed: 35461309
- Li C et al. Adipose Mesenchymal Stem Cell-Derived Exosomes Promote Wound Healing Through the WNT/β-catenin Signaling Pathway in Dermal Fibroblasts. Stem Cell Rev Rep 18:2059-2073 (2022). PubMed: 35471485
- Suo M et al. SS31 Alleviates Pressure Overload-Induced Heart Failure Caused by Sirt3-Mediated Mitochondrial Fusion. Front Cardiovasc Med 9:858594 (2022). PubMed: 35592397