Application
Western blot
Sample
Human Cell lysate - whole cell (VASCULAR)
Gel Running Conditions
Reduced Denaturing (1-15)
Loading amount
15 µg
Specification
VASCULAR
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Diluent: MILK
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-rrabitIgG, HRP-linked Antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ecl
Exposure
2 minute(s) and 0 second(s)
Additional data
Additional Notes
I have tried to use at 1:5000 and 1:1000 without success.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 11 2021