Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen I antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered formalin

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: CY3 donkey anti-rabbit secondary antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®

Additional data

Abcam response
Thank you very much for your valuable feedback. We are pleased that this antibody is working well for you in IHC-P. Currently we are not able to guarantee results in IHC-P with this antibody as we have inconsistent results. However, we would be pleased to receive any further feedback from customers who may have used this antibody in IHC-P with positive or negative results.

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Verified customer

投稿 Sep 25 2015

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