Anti-Collagen I + Collagen III 抗体 (ab24135)
Key features and details
- Rabbit polyclonal to Collagen I + Collagen III
- Suitable for: IHC-Fr, ELISA
- Reacts with: Pig
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Collagen I + Collagen III antibody
Collagen I + Collagen III 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Collagen I + Collagen III -
由来種
Rabbit -
特異性
Specificity is measured by ELISA using pure pig collagen types I and III. No binding to pig serum proteins is seen at similar dilutions of the antibody. No cross reactivity seen against pig basement membrane collagens type IV and V in immunostaining. -
アプリケーション
適用あり: IHC-Fr, ELISAmore details -
種交差性
交差種: Pig -
免疫原
Full length native collagen type I and III from pig skin.
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ポジティブ・コントロール
- Pig kidney and skin. Cultured porcine cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 1ml of distilled water and add preservative if required. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 8.00
Constituents: 0.16% Sodium phosphate, 0.38% Sodium borate, 0.87% Sodium chloride, 1% Dextran, 1% Mannitol -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Pooled antisera are passed over DEAE-cellulose to produce an IgG enriched fraction. Antibodies specific to type I and type III pig collagen are then immobilised by affinity chromatography and eluted with an acidic buffer. The final purification steps are neutralisation, dialysis and lyophilisation. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24135の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
1/20.
|
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ELISA |
1/3000.
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特記事項 |
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IHC-Fr
1/20. |
ELISA
1/3000. |
ターゲット情報
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関連性
Collagen I is a fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with Collagen I. Mutations in this gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons. -
細胞内局在
Secreted -
別名
- Alpha 1 type I collagen antibody
- Alpha 2 type I collagen antibody
- COL1A1 antibody
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab24135 は 1 報の論文で使用されています。
- Luo Q et al. hsa_circ_0001018 promotes papillary thyroid cancer by facilitating cell survival, invasion, G1/S cell cycle progression, and repressing cell apoptosis via crosstalk with miR-338-3p and SOX4. Mol Ther Nucleic Acids 24:591-609 (2021). PubMed: 33898108