Anti-Cofilin (phospho S3) 抗体 [EPR24753-28] (ab283500)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24753-28] to Cofilin (phospho S3)
- Suitable for: WB, IHC-P, Dot blot, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cofilin (phospho S3) antibody [EPR24753-28]
Cofilin-1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24753-28] to Cofilin (phospho S3) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, Dot blot, ICC/IF, Flow Cyt (Intra)more details
適用なし: IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa, 293T, NIH/3T3 and C6 lysates all without alkaline phosphatase treatment. IHC-P: Human breast carcinoma, Human stomach, Mouse colon, Rat colon tissues all without alkaline phosphatase treatment. ICC/IF: HeLa cells without alkaline phosphatase treatment. Flow Cyt: HeLa cell. Dot Blot: Cofilin-1 (phospho S3) peptide.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24753-28 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab283500の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 18 kDa.
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IHC-P |
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Dot blot |
1/1000.
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ICC/IF |
1/1000.
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Flow Cyt (Intra) |
1/600.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 18 kDa. |
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Dot blot
1/1000. |
ICC/IF
1/1000. |
Flow Cyt (Intra)
1/600. |
ターゲット情報
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機能
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. -
組織特異性
Widely distributed in various tissues. -
配列類似性
Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain. -
翻訳後修飾
Phosphorylated on Ser-3 in resting cells. -
細胞内局在
Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. - Information by UniProt
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参照データベース
- Entrez Gene: 1072 Human
- Entrez Gene: 12631 Mouse
- Entrez Gene: 29271 Rat
- Omim: 601442 Human
- SwissProt: P23528 Human
- SwissProt: P18760 Mouse
- SwissProt: P45592 Rat
- Unigene: 170622 Human
see all -
別名
- 18 kDa phosphoprotein antibody
- CFL 1 antibody
- CFL antibody
see all
画像
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All lanes : Anti-Cofilin (phospho S3) antibody [EPR24753-28] (ab283500) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate (Untreated membrane)
Lane 2 : HeLa(human cervix adenocarcinoma epithelial cell), whole cell lysate (Phosphatase treated membrane)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 37 seconds
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All lanes : Anti-Cofilin (phospho S3) antibody [EPR24753-28] (ab283500) at 1/1000 dilution
Lane 1 : 293T (human embryonic kidney epithelial cell), whole cell lysate (Untreated membrane)
Lane 2 : 293T (Phosphatase treated membrane)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 15 seconds
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All lanes : Anti-Cofilin (phospho S3) antibody [EPR24753-28] (ab283500) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate (Untreated membrane)
Lane 2 : NIH/3T3 (Phosphatase treated membrane)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-Cofilin (phospho S3) antibody [EPR24753-28] (ab283500) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate (Untreated membrane)
Lane 2 : C6 (Phosphatase treated membrane)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Cofilin (phospho S3) with ab283500 at 1/5000 (0.121 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human breast carcinoma without alkaline phosphatase treatment (image A). (PMID: 27799793) No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab283500 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Human stomach tissue labelling Cofilin (phospho S3) with ab283500 at 1/5000 (0.121 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human stomach without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab283500 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling Cofilin (phospho S3) with ab283500 at 1/5000 (0.121 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse colon without alkaline phosphatase treatment (image A). (PMID: 15649475) No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab283500 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling Cofilin (phospho S3) with ab283500 at 1/5000 (0.121 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat colon without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab283500 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling Cofilin (phospho S3) with ab283500 at 1/1000 (0.604 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing nuclear and cytoplasmic staining in HeLa cells, no staining in HeLa treated with Alkaline Phosphatase (37°C) for 1 hour is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Cofilin (phospho S3) with ab283500 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Dot blot analysis of Cofilin (phospho S3) using ab283500 at 1/1000 (0.604 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.
Lane 1: Cofilin-1 (phospho S3) peptide
Lane 2: Cofilin-1 non-phospho peptide
Exposure time: 3 minutes
Blocking and diluting buffer and concentration: 5% NFDM/TBST
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab283500 は 1 報の論文で使用されています。
- Ma L et al. SGLT2 inhibitor dapagliflozin reduces endothelial dysfunction and microvascular damage during cardiac ischemia/reperfusion injury through normalizing the XO-SERCA2-CaMKII-coffilin pathways. Theranostics 12:5034-5050 (2022). PubMed: 35836807