Anti-Cofilin 2 (phospho S3) 抗体 (ab14134)
Key features and details
- Rabbit polyclonal to Cofilin 2 (phospho S3)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Cofilin 2 (phospho S3) antibody
Cofilin 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cofilin 2 (phospho S3) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Chicken, Human
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免疫原
Synthetic peptide corresponding to Human Cofilin 2 aa 1-100 (phospho S3) conjugated to keyhole limpet haemocyanin.
Database link: Q9Y281 -
ポジティブ･コントロール
- WB: L6 whole cell lysate treated with LIM kinase.
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特記事項
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14134の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 19 kDa.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 19 kDa. |
ターゲット情報
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機能
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. -
組織特異性
Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues. -
関連疾患
Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved. -
配列類似性
Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain. -
翻訳後修飾
The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal. -
細胞内局在
Nucleus matrix. Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 1073 Human
- Entrez Gene: 12632 Mouse
- Entrez Gene: 366624 Rat
- Omim: 601443 Human
- SwissProt: P21566 Chicken
- SwissProt: Q9Y281 Human
- SwissProt: P45591 Mouse
- Unigene: 180141 Human
see all -
別名
- CFL 2 antibody
- CFL2 antibody
- COF2_HUMAN antibody
see all
画像
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Western blot - Anti-Cofilin 2 (phospho S3) antibody (ab14134)All lanes : Anti-Cofilin 2 (phospho S3) antibody (ab14134) at 4 µg/ml
Lane 1 : L6 lysate in RIPA buffer, untreated
Lane 2 : L6 lysate in RIPA buffer, treated with 100mU LIM Kinase 1 (active)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP)
Developed using the ECL technique.
Predicted band size: 19 kDaL6 lysate was incubated with or without 100 mU LIM Kinase 1 (active) at 30°C for 30 minutes. Phosphorylation was terminated by boiling in reducing sample buffer. Proteins were visualized using a chemiluminescence detection system.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab14134 は 2 報の論文で使用されています。
- Wong SW et al. H2O 2 Exposure Affects Myotube Stiffness and Actin Filament Polymerization. Ann Biomed Eng 43:1178-88 (2015). PubMed: 25371376
- Nworu CU et al. Preparation of developing Xenopus muscle for sarcomeric protein localization by high-resolution imaging. Methods 66:370-9 (2014). PubMed: 23806641
